轻度慢性乙型肝炎患者脾胃湿热证的microRNAs研究

Study on micro RNAs of Spleen-Stomach Damp-Heat Syndrome of Mild Chronic Hepatitis B

目的从micro RNAs调控角度揭示轻度慢性乙型肝炎脾胃湿热证发生的分子生物学机制。方法将符合标准的病例分为轻度慢性乙型肝炎无症状者组、轻度慢性乙型肝炎脾胃湿热证组,另设健康正常人组,空腹采集静脉血,借助Agilent Human mi RNA 8×60k微阵列芯片检测血浆中micro RNAs表达谱,运用SPSS19.0软件及在线SAS系统分析数据,求得各组间的差异表达micro RNAs谱(P<0.05),借助mi RNA生物信息学分析软件Target Scan(V5.2)预测其靶基因并行靶基因功能富集分析。结果轻度慢性乙型肝炎脾胃湿热证的差异表达micro RNAs共60条(P<0.05),26条上调,34条下调;2倍以上差异表达的micro RNAs23条,上调10条,其靶基因共500个,通过GO分析其功能主要涉及转录因子活性、免疫系统的发育、转运活动、细胞粘附等生命过程;下调13条,其靶基因共499个,通过GO分析其功能主要涉及转录因子活性、转移酶的活性、核酸结合、蛋白结合、转运、免疫系统的发育等生命过程。结论轻度慢性乙型肝炎脾胃湿热证存在特异性差异micro RNAs表达谱,涉及多个生命过程,揭示了轻度慢性乙型肝炎脾胃湿热证的分子机制。

Objective To reveals molecular mechanisms of Spleen - Stomach Damp - Heat Syndrome of Mild Chronic Hepatitis B from microRNAs regulating. Methods The patients were divided into mild compliant asymptomatic chronic hepatitis B group, mild chronic hepatitis B with Spleen -Stomach Damp -Heat Syndrome group, healthy people group, fasting serum samples were collected, Expression profile of microRNAs in plasma was detected using Agilent Human miRNA 8 × 60k microarrays, data was analyzed using SPSS19.0 software and online SAS system, to obtain the differences expressed microRNAs spectrum between thegroups （P 〈 0.05 ） ,predicting their target genes and analyzing target miRNAs gene function enrichment using bioinformatics analysis software TargetScan （V5.2）. Results Spleen - Stomach Damp - Heat Syndrome of Mild Chronic Hepatitis B has 60 differentially expressed microRNAs （ P 〈 0.05 ） , including 26 up - regulated,34 down. There were 23 differentially expressed microRNAs （ two times higher） , there were 10 of which raised,its target genes had 500, whose function mainly involved transcription factor activity, immune system development, transshipment, cell adhesion and other life processes through GO analysis of its;there were 13 raised, its target genes had 499, whose function mainly involved transcription factor activity, transferase activity, nucleic acid binding, protein binding, transport, immune system development and other life processes. Conclusion There are specific differences in expression profiles of microRNAs in Spleen - Stomach Damp - Heat Syndrome of Mild Chronic Hepatitis B, involving multiple life processes ,revealing its molecular mechanisms.