家系冠心病血瘀证差异基因的研究分析

Analysis of the gene family of blood-stasis syndrome of coronary heart disease difference

目的探讨冠心病血瘀证家系差异基因的功能及目标通路。方法应用Affymetrix公司的Human Genome U133 Plus2.0芯片检测家系冠心病血瘀证组(A组)、家系冠心病非血瘀证组(B组)、家系非冠心病血瘀证组(C组)、家系健康人组(D组)的基因表达谱,通过基因本体论(Gene Ontology,GO)分析基因功能;通过Biocarta及KEGG通路找到每一个差异基因所在通路。结果家系冠心病血瘀证差异基因主要以炎症因子为主。当P<0.05时研究发现,家系冠心病血瘀证的差异基因表达主要涉及到如下几类基因系的表达改变;1趋化因子;2白介素细胞因子;3基质金属蛋白酶MMPs系;4成纤维细胞生长因子。结论家系冠心病血瘀证相关差异基因,主要涉及到炎症、斑块形成、内皮损伤方面。

Objective To explore the function and target pathway genes of family coronary heart disease of blood - stasis. Meth- ods Detection of family blood stasis syndrome of coronary heart disease group （ group A） , family of coronary heart disease the non blood stasis syndrome group （B group） , the family of non blood stasis syndrome of coronary heart disease group （group C）, fami- ly health group （group D） gene expression profile by Affymetrix HumanGenome U133 Plus 2.0 chip;Analysis of gene function by Gene Ontology ; For each gene the pathway by Biocarta and KEGG. Results Gene family blood - stasis syndrome of coronary heart disease with inflammation factor differences. When P 〈 0.05, the study found, differentially expressed gene family coronary heart disease with blood stasis syndrome mainly involves the following gene expression changes：①The chemotaetic factor ;②Interleukin cytokines;③Matrix metalloproteinase MMPs systemFibroblast growth factor. Conclusion Genetic variations associated with family blood stasis syndrome of coronary heart disease, mainly related to inflammation, plaque formation, endothelial injury.