菝葜不同提取部位对卵巢癌细胞的活性影响

Effect of Different Extracts of Smilax China L. on the Activity of Ovarian Cancer Cells

目的:探讨中药菝葜不同提取物对卵巢癌细胞的活性影响。方法:采用溶剂法利用正丁醇和乙酸乙酯提取菝葜中的有效成分,获得菝葜不同提取部位。采用CCK-8法检测菝葜不同提取物(0,50,100,150,200μg·ml-1)对低侵袭力A2780及高侵袭力HO-8910PM卵巢癌细胞存活率的影响,同时观察不同时间点24,48,72 h两种卵巢癌细胞的状态。结果:正丁醇提取部位(SCR-B)对A2780和HO-8910PM卵巢癌细胞的IC50分别为47.5,69.2μg·ml-1,乙酸乙酯提取部位(SCR-E)对A2780和HO-8910细胞的IC50分别为147.9,166.0μg·ml-1;菝葜提取物对两种卵巢细胞A2780及HO-8910PM均有抑制作用,并呈剂量及时间依赖性。结论:SCR-B对卵巢癌细胞活性的抑制率强于SCR-E,且其对A2780细胞的抑制作用强于HO-8910人卵巢癌细胞。SCR-B对卵巢癌细胞具有更好的抑制作用。

Objective： To investigate the effects of different extracts of Smilax china L on the activity of ovarian cancer cells. Methods：Solvent extraction method was used to extract the active ingredients of Smilax china L. , and CCK-8 assay method was ap-plied to detect the influence of different Smilax china L. extracts （0, 50, 100, 150 and 200μg＆#183;ml-1 ） on the survival rate of ovarian cancer cells including low invasiveness A2780 cells and high invasiveness HO-8910PM cells. At the same time, the status of the two kinds of ovarian cancer cells at different time points （24, 48 and 72 h） was observed. Results：The IC50 of N-butanol extracts （SCR-B） on HO-8910 and A2780 ovarian cancer cells was 47. 5 μg＆#183; ml-1 and 69. 2 μg＆#183; ml-1 , respectively, and that of ethyl acetate ex-tracts （SCR-E） on A2780 and HO-8910 cells was 147. 9 μg＆#183; ml-1 and 166. 0 μg＆#183; ml-1, respectively. Smilax china L. extracts had the inhibition against both A278 and HO-8910PM ovarian cells in a dose-and time-dependent manner. Conclusion：The inhibitory activity of SCR-B against ovarian cancer cells is stronger than that of SCR-E, and SCR-B has stronger inhibition against A2780 cells than against HO-8910 cells. SCR-B has better inhibition against ovarian cancer cells.