天麻钩藤饮中天麻素提取工艺优选

Extraction Process Optimization of Gastrodine in Tianma Gouteng Decoctions

目的:优选天麻钩藤饮中天麻素的提取工艺。方法:以提取物浸膏得率及浸膏中天麻素含量为考察指标,采用正交设计法及高效液相色谱法考察煎煮时间、加水倍数及煎煮次数对天麻素提取效果的影响。结果:提取次数对天麻素的提取率有显著性影响。优选的提取工艺为:12倍量的水提取3次,每次1.5 h。结论:优选得到的工艺可行,可用于以天麻素为质控指标的天麻钩藤饮有效部位的制备。

Objective：To establish an optimal extraction technology for gastrodine in Tianma Gouteng decoctions. Methods： An orthogonal design with extract yield and gastrodine concentration as the indices was carried out to observe the influence of the extraction time, water amount and extract times on the extraction result. Results：The extract times showed significant influence on the extraction percentage of gastrodine. The best extraction parameters were as follows：adding 12-fold water and decocting 3 times with 1. 5h for each time. Conclusion：The established extraction process is feasible, which can be used in the preparation of the effective part for Tianma Gouteng decoctions with gastrodine concentration as the index.