香菇多糖联合氟尿嘧啶体内外抗肝肿瘤作用的研究

In vitro and in vivo Antitumor Effect of Lentinan Combined with 5-FU

目的:研究香菇多糖与氟尿嘧啶(5-FU)的联合抗肝肿瘤作用。方法:采用MTT法检测香菇多糖联合5-FU对H22细胞增殖抑制作用;PI单染法检测细胞周期;接种H22细胞昆明小鼠为模型,进行体内抑瘤作用的考察,测定抑瘤率、脾/胸腺指数以及脾组织细胞因子白介素-2(IL-2)、白介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)浓度。结果:体外研究结果表明,香菇多糖的加入并没有进一步提高5-FU对H22细胞的抑制率,对细胞周期也没有明显的影响;体内抑瘤实验表明,香菇多糖与5-FU具有协同抑瘤作用,与单独使用5-FU比较,香菇多糖25,50 mg·kg-1联合5-FU组抑瘤率显著提高(P<0.05);香菇多糖25、50 mg·kg-1可以纠正5-FU所致的荷瘤小鼠免疫抑制作用,并能提高荷瘤小鼠脾/胸腺指数。结论:香菇多糖与5-FU具有协同抑瘤作用,其机制为非特异性免疫刺激,并非直接杀伤肿瘤细胞。

Objective：To study the in vitro and in vivo anti-tumor effect of lentinan combined with 5-FU. Methods：MTT method was used to determine the proliferation of H22 in vitro, and the cell cycle changes were analyzed by a flow cytometry. The in vivo anti-tumor effect was evaluated in Kunming mice. The tumor inhibitory rate, thymus/spleen index, IL-2, IL-6 and TNF-α were deter-mined. Results：The in vitro results revealed that lentinan had no obvious effect on the inhibitory rate and cell cycle of the cells treated with 5-FU. In vivo results showed that lentinan at the dosage of 25 and 50 mg＆#183;kg-1 could obviously enhance the anti-tumor effect of 5-FU（P〈0. 05）. Furthermore, lentinan could increase the thymus/spleen index of the tumor-bearing mice. Conclusion：Lentinan com-bined with 5-FU has synergistic effects on anti-tumor activity through nonspecific immune stimulation rather than the direct killing of tumor cells.