RP-HPLC法同时测定升麻黄芩颗粒中黄芩苷、芍药苷和葛根素

Simultaneous Determination of Baicalin,Paeoniflorin and Puerarin in Shengma Huangqin Granules by RPHPLC

目的：建立升麻黄芩颗粒中黄芩苷、芍药苷和葛根素的含量测定方法。方法：采用反相高效液相色谱法,色谱柱为Kromasil-C18（150 mm×4.6 mm,5μm）,流动相为乙腈-0.1%磷酸溶液（20∶80）,等度洗脱。采用不同的流速以及波长：在1到12 min内,流速为1.0 ml·min-1,检测波长为230 nm;在12.01 min到20.0 min,流速为1.5 ml·min-1,检测波长为278 nm。柱温为30℃,进样量为20μl。结果：黄芩苷、芍药苷和葛根素的线性范围分别为10～160μg·ml-1、30～480μg·ml-1、20～320μg·ml-1;平均回收率分别为100.17%,100.14%和100.68%,RSD分别为1.29%、0.47%和1.81%。结论：该方法准确、简便、重复性好,可用于升麻黄芩颗粒中黄芩苷、芍药苷和葛根素的定量测定。

Objective：To develop a RP-HPLC method for simultaneously determining the contents of baicalin,paeoniflorin and pu-erarin in Shengma Huangqin granules. Methods：Three active compounds were chromatographically separated on a Kromasil C18 column （150 mm×4.6 mm,5 μm） using the isocratic mobile phase composed of acetonitrile-0.1%H3PO4（ 20∶80） coupled with changed flow rate and detection wavelength：1-12 min：1. 0 ml·min-1 ,230nm;12. 01-20. 0 min：1. 5 ml·min-1 ,278nm. The temperature of column was 30℃,and the injection volume was 20μl. Results：The linear range of baicalin, paeoniflorin and puerarin was 10-160,30-480 μg·ml-1and 20-320 μg＆#183;ml-1 with average recovery of 100. 17%（RSD=1. 29%）,100. 14%（RSD=0. 47%） and 100. 68%（RSD=1. 81%）,respectively. Conclusion： The method is convenient,accurate and reproducible for the determination of baicalin , paeoniflorin and puerarin in Shengma Huangqin granules.