探索学习对脑梗死大鼠缺血侧海马区微血管密度及Flt-1表达的影响

Effects of learning environment on microvessel density and Flt-1 expression in hippocampus of rats after cerebral infarction

目的研究探索学习对局灶性脑梗死大鼠缺血侧海马区微血管密度及Flt-1表达的影响。方法采用开颅电凝法制作SD大鼠右侧大脑中动脉缺血(MCAO)模型,术后24 h大鼠随机分为标准环境组(SE组),探索学习环境组(LE组)。分别于术后1 d、3 d、7 d、14 d、28 d以免疫组织化学法检测海马区微血管密度及Flt-1的表达。结果大鼠大脑中动脉栓塞后,缺血侧海马区微血管密度显示LE组在术后14 d、28 d明显高于SE组大鼠(P<0.05);术后1 d,缺血侧海马区Flt-1表达最高,经探索学习环境干预后Flt-1表达明显增加。结论探索学习可促使缺血侧海马区Flt-1表达上调,进而促进微血管新生,利于学习记忆功能恢复。

Objective To study the effects of learning environment on microvessel density and Flt-1 expression in hippocampus of rats after cerebral infarction. Methods After the models of right middle cerebral artery occlusion（ MCAO）with electric coagulation,the models of SD rats were randomly divided into learning envionment（ LE） and control group（ SE）. The microvessel density and Flt-1 in hippocampus were measured with immunohistochemstry 1、3、7、14 and 28 days after operation. Results The microvessel density in hippocampus of ischemia side in LE was significantly higher than that of rats in SE group at 14 and 28 days after MCAO（ P 0. 05）. The expression of Flt-1 in hippocampus of ischemic side was the highest at 1d after MCAO,especially in LE. Conclusion Learning can promote the expression of microvessel density and Flt-1 in ischemic hippocampal,then promote angiogenesis and improve the learning and memory function recovery.