脑脉泰对ApoE基因敲除小鼠动脉粥样硬化斑块稳定性的影响

The effect of Naomaitai on instable plaque of ApoE knockout mice

目的探讨脑脉泰对ApoE基因敲除小鼠动脉粥样硬化斑块稳定性影响及其机制。方法采用ApoE基因敲除小鼠制备动脉粥样硬化模型,具有相同遗传背景的C57BL/6J小鼠作为正常对照组,分别给予不同剂量的脑脉泰和阿托伐他汀。高脂饲料喂养16 w后HE染色法观察主动脉根部斑块大小,免疫组化法检测动脉斑块MMP-2、MMP-9和巨噬细胞CD68的表达。结果与正常组相比,模型组斑块明显增大,阿托伐他汀组和脑脉泰组斑块面积显著减小且阿托伐他汀组和脑脉泰组斑块大小无显著性差异。免疫组化结果显示与模型组相比,给予脑脉泰药物后斑块处MMP-9、MMP-2和CD68表达显著性下降(P<0.05),但与阿托伐他汀组相比无显著性差异。结论脑脉泰通过降低MMP-9、MMP-2和CD68的表达减少不稳定斑块的破损。

Objective To study the effect of Naomaitai on instable plaque and and potentional mechanism in Apo Eknockout mice. Methods Apo E-knockout mice were used to establish the modle of atherosclerosis and C56 BL /6J mice were used as the control group. Then they were given different doses of atorvastatin or Naomaitai and continuously fed with high-fat diet for 16 weeks. Aortic root was prepared for paraffin slice. Histomorphometric analysis of atherosclerotic lesion was performed by means of HE,relative MMP-2,MMP-9,CD68 expression were performed by immunohistochemistry.Results Compared with model group,both Naomaitai group and atorvastatin group showed smaller area of plaque,but there was no markedly differences between the two treated goup. Expression of MMP-9,MMP-2,CD 68 on plaques were decreased in group and Atorvastatin group. Conclusion Naomaitai might make plaques stable by inhibiting expressions of MMP-9,MMP-2 and CD68.