PCR检测食品中金黄色葡萄球菌肠毒素基因

PCR Detection on Staphylococcus aureus Entertoxin Genes in Food

目的:检测食品中金黄色葡萄球菌肠毒素基因谱携带情况,探讨与食物中毒发病相关的金黄色葡萄球菌基因类型。方法:取食品中检出的金黄色葡萄球菌进行分离培养鉴定,通过PCR技术检测样本18种不同型的肠毒素基因的携带情况,并进行统计学分析。结果:食品中检出的金黄色葡萄球菌肠毒素基因有较高的阳性检出率,主要检出sea、seb、sei、seg、sel、sem、sen等基因,其中sea基因检出率最高,达到77.27%;sei、seg、sem、sen基因具有相关性,检出率为9.09%;seb、sel基因的检出率为4.55%。结论:在食品中主要检出金黄色葡萄球菌肠毒素sea、sei、seg、sem、sen、seb、sel等基因,其中,sei、seg、sem、sen基因的相关性还有待进一步深入研究。

[Objective] To investigate whether the presence of any of the 18 kinds of currently known Staphylococcus aureus enterotoxin genes could be correlated with food samples. [Method] The Staphylococcus aureus stains were taken from food samples, and the presence of the 18 kinds of currently known enterotoxin genes were systematically tested by PCR. [Result] Higher positive rases of Staphylococcus aureus enterotoxin genes were detected. The most frequently detected enterotoxin genes were sea, seb, sei, seg, sel, sem, and sen. Of the total strains studied, 77.27% contained sea. The detectable rate of sei, seg, sem and sen were 9. 09% and that of seb and sel were 4. 55%. [Conclusion] The enterotoxins gene sea, sei, seg, sem, sen, seb, and sel might play roles in food samples, further research will be nee- ded to provide new insights between sei, seg, sem and sen.