商陆皂苷甲对BXSB小鼠狼疮肾炎凋亡的影响

Effect of esculentoside A on apoptosis of lupus nephritis-prone BXSB mouse

目的通过观察商陆皂苷甲(Es A)对BXSB小鼠肾脏细胞凋亡的影响,探讨Es A对狼疮肾炎的作用机制。方法将24只18周龄雄性BXSB小鼠随机分为模型组、地塞米松组和Es A组,腹腔注射相应药物治疗4周后留取肾组织标本,采用原位末端标记法检测肾脏细胞凋亡情况,免疫组化检测组织中Bax、Bcl-2、Fas、Fas L表达情况。结果 13组间肾小球和肾小管细胞的凋亡指数差异有统计学意义(P<0.05),Es A组凋亡指数最高,其次为地塞米松组。2与模型组比较,两治疗组小鼠肾组织Bcl-2光密度值显著下降(P<0.05),Es A和地塞米松治疗组间无统计学差异(P>0.05);3组间Bax的表达比较差异无统计学意义(P>0.05);而3组间肾组织Bcl-2/Bax的比值比较差异有统计学意义(P<0.05)。3与模型组比较,两治疗组BXSB小鼠的肾组织Fas、Fas L光密度值明显增加(P<0.05),Es A和地塞米松治疗组间差异无统计学意义(P>0.05)。结论 Es A具有诱导BXSB小鼠肾小球和肾小管细胞凋亡作用,其通过下调BXSB小鼠肾组织中Bcl-2表达及上调Fas、Fsa L表达而促进细胞凋亡的发生。

Objective It is to observe the effects of esculentoside A（ Es A） on the renal cell apoptosis of BXSB mouse,and explore the mechanism of ESA on Lupus nephritis. Methods 24 cases of male BXSB mice at the age of 18 weeks were randomly divided into model group,Es A group and Dexamethasone group. The BXSB mice were treated by Sodium Chloride,ESA and Dexamethasone. Four weeks later,all mice were killed to obtain nephridial tissue. The apoptosis ratio of nephros cells was detected by in situ end labeling method,the expression of Bax,Bcl-2,Fas and Fas L in nephridial tissue were detected by Immunohistochemical method. Results 1There was significant difference in apoptosis index of nephros cells among three groups（ all P〈0. 05）. The apoptosis index of Es A group was highest and Dexamethasone group was following. 2The expression of Bcl-2 in kidneys of model group was significantly higher than that of Es A and Dexamethasone group（ P〈0. 05）; there was no significant difference between Es A and Dexamethasone group（ P〈0. 05）. There was no significant difference in the express of Bax among three groups（ all P〈0. 05）. There was significant difference among there groups in the ratio of Bcl-2 / Bax（ all P〉0. 05）. 3The expression of Fas and Fas L in kidneys of model group was significantly lower than that of Es A and Dexamethasone group（ P〉0. 05）,there was no difference between Es A and Dexamethasone group（ P〉0. 05）.Conclusion Es A can degrade urine protein concentration and improve renal function,pathological kidney damage in BXSB mice. Es A can promote the apoptosis of nephros cell,and down regulation the expression of Bcl-2 and up regulation the expression of Fas and Fas L in BXSB mice.