摘要
利用水热法合成了三元配合物[Ni(pht)2(pn)2](Hpht:苯妥英;pn:1,2-丙二胺),通过电子吸收光谱法、溴化乙锭(EB)荧光分析法以及粘度测定研究了此种配合物与DNA的作用。结果表明:此种配合物与DNA作用时,其电子吸收光谱的最大吸收峰产生减色效应,配合物能猝灭EB-DNA体系的荧光,同时,配合物也能使DNA体系的粘度增大,表明此种配合物与DNA存在插入结合作用。
The title complex [Ni（ pht）2（ pn）2]（ Hpht： 5,5-Diphenylhydantion）; pn： 1,2-propanediamine） was synthesized by hydrothermal method. The interaction of the complex and calf thymus DNA（ CT-DNA） was studied by electronic absorption,ethidium bromide（ EB） fluorescence spectroscopy and viscosity measurements. The result indicated that the maximal absorption intensity of this complex was weakened; At the same time,it can to some extent quench the emission intensity of EB-DNA system and viscosity was increased. The interaction between the complex and CT-DNA was confirmed the effect of intercalation complex into DNA double helix.
出处
《合成材料老化与应用》
2015年第1期59-62,共4页
Synthetic Materials Aging and Application
基金
国家自然科学基金(No.21101069/B0104)
国家海洋公益性行业科研专项(201305000)
淮海工学院大学生实践创新训练项目(No.5509013)
