摘要
目的:通过观察参芪补心丸对心肌缺血模型大鼠ICAM-1,VCAM-1mRNA表达的影响,探讨参芪补心丸治疗冠心病的作用机制和疗效。方法:健康Wistar大鼠60只,雌雄各半。将其随机分为空白对照组、模型对照组、心通口服液组、参芪补心丸高、中、低剂量组,每组10只。利用原位杂交技术观察参芪补心丸对冠心病模型大鼠ICAM-1,VCAM-1mRNA表达的影响。结果:与空白对照组比较,除参芪补心丸高剂量组其余各组大鼠心肌组织ICAM-1和VCAM-1mRNA含量增高,有极显著差异(P<0.01);与模型对照组比较,参芪补心丸高、中剂量组均可降低大鼠心肌组织ICAM-1和VCAM-1mRNA含量,有极显著差异(P<0.01),心通口服液组有显著差异(P<0.05);与心通口服液组比较,参芪补心丸高剂量组ICAM-1的含量降低,有极显著差异(P<0.01),中剂量组ICAM-1含量降低,有显著差异(P<0.05);与心通口服液组比较,参芪补心丸高、中剂量组VCAM-1mRNA的含量降低,有极显著差异(P<0.01);与参芪补心丸中、低剂量组比较,参芪补心丸高剂量组ICAM-1的含量降低,有极显著差异(P<0.01)。结论:参芪补心丸通过降低心肌缺血模型大鼠ICAM-1,VCAM-1mRNA的表达,与对照组相比能够明显减轻血管内皮炎性反应,抗血管内皮损伤。
Objective: To observe the influence of Shenqi Buxin Pill on ICAM- 1 and VCAM- 1mRNA of the rat model of coronary heart disease and to study the mechanism and effect of Shenqi Buxin Pill. Methods: 60 rats were randomly divided into normal group,model group,Xintong Oral Liquid group and high dose of Shenqi Buxin Pill group,middle dose of Shenqi Buxin Pill group,low dose of Shenqi Buxin Pill group,10 rats in each group. Using in situ hybridization,we observed the influence of Shenqi Buxin pill on ICAM- 1 and VCAM- 1mRNA level of the rat model of coronary heart disease. Results: Comparing with the control group,in addition to the high dose group of Shenqi Buxin Pill,there was increased expressions of ICAM- 1 and VCAM- 1mRNA of myocardial tissue of rats in other groups( P〈 0. 01). Comparing with model group,the high and middle dose groups of Shenqi Buxin Pill all can reduce the expressions of ICAM- 1and VCAM- 1mRNA of myocardial tissue of rats and there were differences( P〈 0. 01). At the same time,it had difference comparing model group and Xintong Oral group( P〈 0. 05). Comparing with Xintong Oral group,the high dose group of Shenqi Buxin Pill can reduce the content of ICAM- 1,also,there was difference( P〈 0. 01) and the middle dose group of Shenqi Buxin Pill can reduce it too. At the same time,there was difference between them( P〈 0. 05).Comparing with Xintong Oral group,the high and middle dose groups of Shenqi Buxin Pill can reduce the content of VCAM- 1mRNA and there was difference( P〈 0. 01). Comparing with the middle and low dose groups of Shenqi Buxin Pill,the high dose group can reduce the contents of ICAM- 1 and VCAM- 1mRNA obviously and it had difference( P〈 0. 01). Conclusions: Shenqi Buxin Pill can reduce the expressions of ICAM- 1 and VCAM- 1mRNA in rats model of coronary heart disease and it can reduce the endothelial inflammatory reaction obviously and prevent vascular endothelial in-jury comparing with the control group.
出处
《中华中医药学刊》
CAS
北大核心
2015年第3期673-675,共3页
Chinese Archives of Traditional Chinese Medicine
基金
黑龙江省自然科学基金项目(D201164)