双花抗病毒口服液的质量标准研究

Study on Quality Standard for Shuanghua Kangbingdu Oral Solution

目的：建立双花抗病毒口服液的质量标准。方法：采用薄层色谱法对双花抗病毒口服液中的金银花、野菊花、连翘和广藿香进行定性鉴别;以高效液相色谱法对其金银花和野菊花中的绿原酸进行含量测定,对其连翘中的连翘苷进行含量测定。结果：双花抗病毒口服液的金银花、野菊花、连翘和广藿香薄层色谱中,在与对照药材相应的位置上显相同颜色的斑点;绿原酸检测浓度在0.011 2-0.089 6 mg·m L-1范围内与峰面积呈良好线性关系（r=0.999 2）,平均回收率为100.7%（RSD=1.17%）;连翘苷检测浓度在0.010 5-0.168 mg·m L-1范围内与峰面积呈良好线性关系（r=0.999 8）,平均回收率为99.6%（RSD=1.00%）。结论：所建质量标准可用于本品的质量控制。

Objective： To establish a method for quality control of Shuanghua Kangbingdu Oral Solution. Methods： TLC was used to identify Flos Lonicere,wild chrysanthemum,forsythia and Pogostemon cablin（ Blanco） Benth. HPLC was used to determine the contents of chlorogenic acid and forsythin. Results： The TLC of Flos Lonicere,wild chrysanthemum,forsythia and Pogostemon cablin（ Blanco） Benth spots with the same color were identified in the corresponding positions.The linear range of chlorogenic acid was 0. 011 2 - 0. 089 6 mg·m L- 1（ r = 0. 999 2）. The average recovery was 100.7% and RSD was 1. 17%. The linear range of forsythin was 0. 010 5 - 0. 168 mg·m L- 1（ r = 0. 999 8）. The average recovery was 99. 6% and RSD was 1. 00%. Conclusion： The method was simple and accurate with strong specificity and could be used to control the quality of Shuanghua Kangbingdu Oral Solution.