人乳头瘤病毒16型E6和E7癌基因对鸡前脂肪细胞增殖的影响

Effect of HPV-16 E6 and E7 oncogenes on chicken preadipocyte proliferation

人乳头瘤病毒的16型E6和E7癌基因已广泛用于建立哺乳动物的永生化细胞,但能否用于建立鸡的永生化细胞尚不清楚。试验究克隆人乳头瘤病毒16型(HPV16)E6和E7癌基因,分别构建这两个病毒癌基因的逆转录病毒表达载体,包装成逆转录病毒颗粒,感染鸡前脂肪细胞后,采用MTT比色法检测E6和E7基因对鸡前脂肪细胞增殖的影响。结果表明,与对照组相比,E7基因显著促进鸡前脂肪细胞增殖,而E6基因则作用不明显。本研究为E6和E7基因在鸡细胞永生化研究奠定基础。

Human papillomavirus type 16 E6 and E7 oncogenes are widely used in the establishment of immortalized mammalian cells, but it is unclear whether E6 and E7 oncogenes can used for immortalizing chicken cells. In the present study, HPV- 16 E6 and E7 genes were cloned and inserted into the retroviral expression vectors. E6 and E7 retroviral expression vectors were packaged into infectious virus particles, respectively. The primary chicken preadipocytes were infected by the retroviruses expressing E6 and E7, respectively. The proliferation of chicken preadipocytes was detected by MTT assay. The results showed that E7 gene significantly promoted chicken preadipocyte proliferation compared with the control group, but E6 gene had no obvious effect on chicken preadipocyte proliferation. Our findings lay the foundation for chicken cell immortalization by E6 and E7 gene.