高血糖后代父系和母系糖脂代谢障碍的差异研究

Investigation on paternal and maternal differences of glycolipid metabolism disorder in hyperglycemia SD rat offsprings

目的探讨糖尿病合并妊娠和妊娠期糖尿病(GDM)的SD大鼠后代中父系和母系糖脂代谢障碍的差异。方法分别把SD大鼠用STZ小剂量腹腔注射和STZ加高糖高脂饮食诱导成GDM和糖尿病合并妊娠模型,得到F1代大鼠后,分别与正常异性8周大鼠杂交产生F2代,分为对照组、GDM母系组、GDM父系组、糖尿病合并妊娠母系组与糖尿病合并妊娠父系组。测定F2代SD大鼠体质量、血糖、胰岛素、三酰甘油及瘦素水平。结果 <1周时,GDM父系组和GDM母系组体质量差异有统计学意义(P<0.05);8周时,糖尿病合并妊娠父系组和糖尿病合并妊娠母系组体质量差异有统计学意义(P<0.05);<1周时的糖尿病合并妊娠组和8周时GDM组,父系组和母系组体质量差异均无统计学意义(P>0.05)。8周时,糖尿病合并妊娠父系组和糖尿病合并妊娠母系组的胰岛素水平、三酰甘油水平差异有统计学意义(P<0.05),空腹血糖水平、瘦素水平差异均无统计学意义(P>0.05)。GDM父系组与GDM母系组的空腹血糖水平、胰岛素水平和三酰甘油水平差异有统计学意义(P<0.05),瘦素水平差异无统计学意义(P>0.05)。结论糖尿病大鼠的糖脂代谢障碍存在父系和母系差异,糖尿病合并妊娠性成熟期和GDM组出生时体质量差异父系组显著高于母系组。糖尿病合并妊娠母系组和GDM母系组TG水平的代谢障碍可能更明显。子代代谢障碍可能与宫内高血糖环境有关。

Objective To investigate the paternal and maternal differences of glycolipid metabolism disorder in the offsprings of SD rats with diabetic pregnancy and gestational diabetes mellitus (GDM).Methods The GDM or diabetic pregnancy models of SD rats were in-duced by intraperitoneal injection of a small dose of STZ or STZ combined with high-sugar and high-fat diet,and then their F1 generation rats were hybridized with normal heterosexual rats of eight weeks to produce the F2 generation hybrid rats,which were divided into five groups:control group,GDM maternal group,GDM paternal group,diabetic pregnancy maternal group,and diabetic pregnancy paternal group.The body mass,levels of blood glucose,insulin,triglyceride and leptin of the F2 generation SD rats in the five groups were detected and compared.Results At <1 week after grouping,the difference in the body mass between the GDM paternal group and the GDM mater-nal group was statistically significant (P<0.05 ),while at 8 weeks,the difference in the body mass between the diabetic pregnancy paternal group and the diabetic pregnancy maternal group also became statistically significant (P<0.05 ).But in the diabetic pregnancy groups at <1 week and the GDM groups at 8 weeks,comparison with body mass between paternal and maternal groups showed no significant difference (P>0.05 ).At 8 weeks,there had significant differences in the insulin level and triglyceride level between the diabetic pregnancy paternal group and the diabetic pregnancy maternal group (P<0.05 ),with no significant differences in the fasting blood glucose level and leptin level between the two groups (P>0.05 ).The differences in the fasting blood glucose level,insulin level,and triglyceride level between the GDM paternal group and the GDM maternal group were significant (P<0.05 ),but not in the leptin level (P>0.05 ).Conclusion There is pa-ternal and maternal differences of glycolipid metabolism disorder in diabetic rats.The paternal and maternal differences of body mass in the diabetic pregnancy groups within sexual maturity period and the GDM groups within newborn period were significant.The metabolic disorder of triglyceride level in the diabetic pregnancy maternal group and the GDM maternal group may be more obvious.The offspring metabolic dis-orders may be associated with intrauterine environment of hyperglycemia.