CD133在直肠癌组织中的表达及与临床病理参数的相关性

The expression of CD133 in rectal cancer and the correlations with clinical pathological characteristics

目的通过检测直肠癌组织CD133的表达并分析其与临床病理参数的相关性,探讨其在临床应用中的价值。方法免疫组化法检测116例直肠癌与癌旁切缘正常组织中CD133蛋白的表达,分析两组间的差异以及癌组织中CD133与临床病理参数性别、年龄、肿瘤直径、分化程度、浸润深度、淋巴结转移与否、p TNM分期的相关性。结果直肠癌组织与癌旁组织中CD133的阳性表达率分别为66.4%、8.6%,两者差异有统计学意义(P<0.05),癌组织中CD133高表达阳性率为33.6%,低表达阳性率为32.8%;癌组织CD133高表达与组织分化程度、淋巴结转移与否呈正相关,相关系数分别为0.453、0.408,与肿瘤直径、肿瘤浸润深度、临床分期无显著相关。结论直肠癌组织中CD133阳性表达率在一定程度上体现直肠癌生物学行为,可作为直肠癌恶性程度判断的新的分子指标应用于病情的评估。

Objective To detect the expression of CD133 in rectal cancer and analyze their correlations with the clinical pathological characteristics,and to investigate the clinical application value. Methods Immunohistochemical staining was performed to detect the expression of CD133 in 116 rectal cancer tissue samples and 116 tissues in the surgical margin of cancer. Analyze the difference between the 2 teams and the correlation with clinical pathological characteristics（ gender,age,tumor size,differentiation degrees,invasion depth,lymph nodes metastasis and p TNM periodization）. Results The positive expression rates of CD133 in rectal cancer tissues was significantly higher than that of surgical margin rectal tissues（66. 4% vs 8. 6%）,the difference between them was statistically significant（ P 0. 05）. The rates of high expression positive and low expression positive were 33. 6% and 32. 8% respectively. There were positive correlations between the expression of CD133 and the degree of differentiation and the lymph node metastases（ r = 0. 453,0. 408）,which had no significant correlation with tumor size,invasion depth,and clinical periodization. Conclusion The expression of CD133 in rectal cancer tissues reflects the biological conduct to a certain degree,it can judge the grade malignancy for rectal cancer as a novel molecular mark.