联合检测胸水脱落细胞Survivin基因及血清Survivin抗体在恶性胸腔积液诊断中的意义

Significance of the combined detection of survivin gene of exfoliative cells in pleural effusion and serum survivin antibody in diagnosis of malignant pleural effusion

目的:探讨胸水Survivin基因及血清Survivin抗体诊断恶性胸腔积液的可行性。方法:选择恶性胸腔积液患者50例(非小细胞肺癌胸水组)及结核性胸腔积液患者50例(对照组),检测并比较2组胸水脱落细胞Survivin基因表达水平和血清Survivin抗体水平。结果:恶性胸水组脱落细胞Survivin基因表达阳性患者12例(24%)明显高于对照组(0%),恶性胸水组血清Survivin抗体浓度(102.10±94.53)pg/m L明显高于对照组(56.32±19.37)pg/mL(P<0.01)。当血清Survivin抗体浓度>80 pg/m L时,对癌性胸水诊断的敏感性及特异性均较好,分别为88.0%和84.6%。结论:胸水脱落细胞Survivin基因联合血清Survivin抗体检测在肺癌性胸腔积液诊断中有一定的临床意义。

Objective： To explore the feasibility of combined detection of surviving gene and serum suvivin antibody in diagnosis of malignant pleural effusion（ PE）. Methods： Fifty cases with malignant pleural effusion（ non-small-cell lung carcinoma pleural effusion group） and 50 cases with tuberculosis pleural effusion（ control group） were involved in the study.The expression of survivin gene of sputum exfoliative cells and serum level of survivin antibody in two groups were detected and compared. Results： Survivin gene showed positive in 12 cases（ 24%）,which was higher than that of control group（ 0%）. The concentrations of survivin antibody in malignant PE were（ 102. 10 ± 94. 53） pg/m L,which was higher than that in the control group（ 56. 32 ± 19. 37） pg / m L）（ P 〈0. 01）. When Survivin antibody concentration exceeded 80 pg / m L,the sensitivity in diagnosing pleural effusion was 88. 0% and specificity was 84. 6% respectively. Conclusions： The combined detection of survivin gene of exfoliative cell in pleural effusion and serum survivin antibody has some clinical significance in diagnosis of lung cancer with pleural effusion.