皮质抑素对脓毒症大鼠心肌凋亡的影响

Effect of cortistatin on myocardium apoptosis in rats with sepsis

目的:探讨皮质抑素对脓毒症大鼠心肌凋亡的影响。方法:SD雄性大鼠随机分为假手术对照组、脓毒症组、皮质抑素+脓毒症组。分别在盲肠结扎穿孔手术后18 h监测平均动脉压及心脏功能,腹主动脉取血处死大鼠,留取血清检测乳酸,留取心肌组织检测丙二醛(MDA)含量、caspase-3活性及p-JAK2、p-STAT3蛋白表达情况,病理切片进行TUNEL染色。结果:脓毒症大鼠表现出平均动脉压降低,心脏功能抑制和高乳酸特征,且心肌caspase-3活性升高,凋亡细胞数目增多及p-JAK2、p-STAT3蛋白表达水平升高。而预先给予皮质抑素可以改善脓毒症大鼠血流动力学及代谢特点,减轻心肌凋亡。结论:皮质抑素可以改善脓毒症介导的心肌凋亡,且这一作用与JAK2/STAT3信号通路相关。

Objective： To investigate the effect of cortistatin on myocardium apoptosis in rats with sepsis. Methods：Rats were randomly divided into 3 groups： control group,sepsis group and cortistatin ＋ sepsis group. Mean arterial pressure（ MAP） and cardiac function were monitored 18 hours after CLP. Then blood was taken from the aortaventralis to sacrifice the rat and determined serum lactic acid. Myocardial MDA content was measured and caspase-3 activity was determined by use of a colorimetric assay kit. Then western blot was used to test p-JAK2 and p-STAT3 protein expression. TUNEL staining was used to measure myocardial apoptosis. Results： Rats with sepsis exhibited a decreased MAP,inhibited heart function and characteristic of high lactic acid. The level of caspase-3 activity,number of myocardium apoptosis and the protein levels of p-JAK2 and p-STAT3 in rats with sepsis were elevated. Rat,pretreated with cortistatin,showed attenuation of blood flow dynamics,and metabolic anormalies as well as reduction of myocardium apoptosis induced by sepsis. Conclusions： Cortistatin may improve the myocardium apoptosis induced by sepsis by inhibiting JAK2 / STAT3 signaling pathway.