肿节风对白血病CEM细胞的抑制作用

Research on the inhibiting function and mechanism of Sarcandra Glabra aqueous extract to CEM cells

目的:研究肿节风水提物对白血病CEM细胞的抑制作用及其机制。方法:以白血病CEM细胞作为研究对象,采用MTT比色试验检测肿节风水提物对CEM细胞的抑制率;光镜、电镜技术观察肿节风水提物作用CEM细胞后其形态的改变;DNA琼脂糖凝胶电泳检测肿节风水提取物对白血病CEM细胞的凋亡作用。结果:肿节风水提物能明显抑制CEM细胞的生长,生药浓度在1.79mg/ml作用24h后,即具有显著的抑制作用,抑制率随剂量的加大和时间的延长而增强,作用48h的半数抑制生药浓度(IC50)约为24.2mg/ml;与对照组相比,实验组光镜、电镜下可见细胞体积缩小,胞浆浓缩、细胞膜皱缩,核解聚、染色质明显浓缩、聚集,边聚于核膜下,甚至形成凋亡小体;琼脂糖凝胶电泳结果显示实验组出现明显的DNA梯形凋亡带,而对照组没有出现梯形条带。结论:肿节风水提物对白血病CEM细胞的生长具有明显的抑制作用,诱导细胞凋亡可能是其抑制白血病CEM细胞生长的机制之一。

Objective：To research the inhibting function and potential mechanism of Sarcandra Glabra Aqueous Extract to the CEM cells. Meth- ods： Using leukemia CEM cells as examination objects, apply MTY to detect the inhibition ratio of Sarcandra Glabra on CEM cells ; apply light microscopy and electron microscopy to observe the structure change of CEM cells ; apply DNA Agarose gel electrophoresis to detect the indu- cing apoptosis effects of Sarcandra Glabra water extract on CEM cells. Resulls：Sarcandra Glabra Aqueous Extract can inhibit the growth of CEM cells obviously when the drug concentration reaches 1.79mg,/ml after 24h and its inhibition ratio grows with the dose increasing and time prolonging; the IC50 is about 24.21mg/ml; in the light microscope and electron microscope, the cells＇volume shrinked , membrane wrinkled, chromosome condensed and gathered under the karyotheca and formed apoptotic body; the Agarose gel electrophoresis results show the clear apoptotic DNA ladder in experimental group while the matched group is on the contrary. Coneluslon：Sarcandra Glabra Aqueous Extract can inhibit the gorowth of CEM cells strongly. To induce apoptosis is one of its mechanisms.