松龄血脉康胶囊对卡马西平在戊四唑诱导癫痫小鼠体内药动学的影响

Effects of Songlingxuemaikang capsules to the pharmacokinetics of carbamazepine in pentylenetetrazol(PTZ)-kinding seizures mice

目的:研究松龄血脉康胶囊对卡马西平在戊四唑诱导癫痫小鼠体内药动学过程的影响。方法:将雄性小鼠按预先设定的时间腹腔注射戊四唑,待癫痫模型制备完毕以后,将小鼠随机分为2组,分别灌胃给予卡马西平(120mg/kg)、卡马西平(120mg/kg)+松龄血脉康(900mg/kg)。在不同时间点采集小鼠血样和脑组织样品,经过处理后通过HPLC测定卡马西平血药浓度和脑组织浓度,绘制药-时曲线图。采用DAS2.0统计学软件计算主要的药动学数据:t1/2β、AUC0-t、CL、Cmax、Tmax。结果:与给戊四唑诱导癫痫小鼠灌胃给予卡马西平相比,联合给予松龄血脉康(900mg/kg)后,卡马西平最大血药浓度(Cmax)增加了17.5%,Tmax提前了35.0%(P<0.05),其他血浆药动学参数无显著性变化;小鼠脑组织药动学参数改变明显:AUC0-t、Cmax分别增加78.9%和52.9%,清除率(CL)下降44.7%,Tmax则缩短了34.6%(P<0.05)。结论:松龄血脉康(900mg/kg)可以影响卡马西平在戊四唑诱导癫痫小鼠体内的药动学过程。建议当松龄血脉康胶囊与卡马西平联用时,应加强临床观察,必要时监测卡马西平血药浓度或降低卡马西平的用量。

Objective： To investigate the effects of Songlingxuemaikang capsules on the pharmacokinetics of carbamazepine in pentylenetetrazol （PTZ） induced mice. Methods ：PTZ was intraperitoneaUy injected into Kuming mice to make the model of conndsion. Then the mice were divided into two groups, and received carbamazepine（120mg/kg） orally alone or combined with Songlingxuemaikang capsules（900mg/kg）. Blood and brain tissue samples were collected at different time. After the samples were pretreated, they were detected by HPLC, and the con- centration -time curves were obtained. The pharmacokinetic parameters including t1/2 β, AUC, CI, Cmax, Tmax were calculated using DAS 2.0. Results： Songlingxuemaikang capsules（900mg/kg） could change the pharmacokinetic parameters of carbamazepine in PTZ-kinding sei- zures mice. The capsules increased the Cmax by 17.5% and shortened Tmax by 35.0% （ P 〈 0.05 ） in plasma, and increased the AUC0.4, Cmax by 78.9% and 52.9%, decreased CL, Tmax by 44.7 % and 34.6% （ P 〈 0.05） in brain, respectively. Conclusion： Songlingxuemai- kang capsules（900mg/kg） could change the pharmacokinetic parameters of carbamazepine in PTZ-kinding seizures mice. Therefore, it is ad- visable to enhance clinical observation.