高压氧暴露对卵巢早衰大鼠骨碱性磷酸酶和抗酒石酸酸性磷酸酶的影响

Effects of hyperbaric oxygen on bone alkaline phosphatelse and tartrate-resistant acid phosphatase in rats with premature ovary failure

目的 探讨卵巢早衰大鼠骨代谢变化及高压氧（HBO）暴露的影响.方法 将40只SPF级雌性Wistar大鼠按数字表法随机分为空白组、造模空白组、雌孕激素替代治疗（HRT）组、HBO组,每组10只.除空白组外,其余各组均给予雷公藤多甙片溶液灌胃,建立卵巢早衰模型.然后,HRT组给予雌孕激素灌胃治疗,HBO组给予HBO治疗及等量生理盐水灌胃,空白组及造模空白组给予等量生理盐水灌胃.观察各组血清骨碱性磷酸酶（BALP）、抗酒石酸酸性磷酸酶（TRACP）的变化.结果 与空白组相比,造模空白组BALP[（245.24±25.21） ng/L]显著降低（P＜0.01）,TRACP[（3 453.69±413.82）pg/L]则显著升高（P＜0.01）;HRT组和HBO组的BALP[分别为（342.16±18.68）、（306.64±22.96）ng/L]均高于造模空白组,TRACP[（1 735.48±94.56）、（1 889.63 ±70.81） pg/L]均低于造模空白组[（3 453.69±413.82） pg/L],差异均有统计学意义（P＜0.01）.结论 HBO暴露可影响卵巢早衰大鼠骨代谢,对促进骨形成的BALP有增强作用,对促进骨吸收的TRACP有抑制作用.

Objective To investigate changes of bone metabolism in rats with premature ovary failure （POF） and the effects of hyperbaric oxygenation （HBO） on bone metabolism.Methods Forty SPF Wistar rats were randomly divided into 4 groups：the blank control group,the model control group,the hormone replacement therapy （HRT） group and the HBO group,each consisiting of 10 animals.Except the blank control group,all the other groups were orally given triptegium wilfordii polyglycisidium （TWP） solution to establish premature ovary failure model.And then,rats in the HRT group were given orally estrogen and progestin.Rats in the HBO group were treated with HBO and the same dosage of normal saline.Rats in the control group and the model control group were given orally the same dosage of normal saline.Then,changes of serum bone alkaline phosphatase （BALP） and tartrate-resistant acid phospatase （TRACP） in different groups were observed accordingly.Results When compared with that of the blank control group,the level of BALP in the model control group[（245.24 ± 25.21） ng/L] was decreased significantly （P 〈 0.01）,while the level of TRACP[（3453.69 ± 413.82） pg/L] was increased markedly （P 〈 0.01）.BALP levels of the HRT group [（342.16 ± 18.68） ng/L] and the HBO group [（306.64 ± 22.96） ng/L] were all higher than those of the model control group,while TRACP levels[（1735.48 ± 94.56） and （1889.63 ± 70.81） pg/L] were all lower than those of the model control group [（3453.69 ± 413.82）pg/L],with statistical significance （P 〈 0.01）.Conclusions HBO could affect bone metabolism in rats with premature ovary failure,had enhancing effect on BALP in bone formation,and at the same time had certain suppressing effect on TRACP in its role of bone absorption.