高压氧对皮瓣移植术后大鼠高迁移率族蛋白B1及中性粒细胞弹性蛋白酶的影响

Effects of hyperbaric oxygen on the levels of high mobility group protein B1 and neutrophil elastase in rats after skin flap transplantation

目的 探讨高压氧（hyperbaric oxygen,HBO）治疗对皮瓣移植后大鼠缺血再灌注损伤炎症反应的影响.方法 40只SD大鼠按随机数字表法分为5组：假手术组（SH组）、缺血再灌注3、5d组（IR组）、HBO治疗3、5d组（HBO组）.建立腹部带蒂移植皮瓣动物模型,予HBO治疗,应用ELISA法检测各组大鼠血清中性粒细胞弹性蛋白酶（neutrophil elastase,NE）及高迁移率族蛋白B1（high mobilitygroup box 1,HMGB1）水平.结果 IR 3、5d组NE水平[（14.26±5.27） mg/L、（10.35±2.53） mg/L]明显高于SH组[（5.25±1.35）mg/L、（5.27±1.84） mg/L] （P ＜0.01）,HBO治疗3、5d组NE水平[（9.42±3.25） mg/L、（8.07 ±2.17） mg/L]明显低于相应的IR组（P＜0.05）.IR 3、5d组HMGB1水平明显高于SH组（P＜0.01）;HBO治疗3d组HMGB1水平明显低于相应IR组（P＜0.01）;HBO治疗5d组HMGB1水平低于相应IR组（P＜0.05）.结论 HBO治疗可显著下调皮瓣移植术后大鼠NE、HMGB1水平,减轻移植术后缺血再灌注损伤造成的炎症反应,提高皮瓣成活率.

Objective To explore the effects of hyperbaric oxygen （HBO） on inflammatory reactions induced by ischemia-reperfusion lesion in rats following skin flap transplantation.Methods Forty SpragueDawley rats were randomly divided into 5 different groups：the sham group,the ischemia-reperfusion （IR） 3-day group （or the IR 3-day group）,the IR 5-day group,the HBO 3-day group and the HBO 5-day group.Abdominal pedicle skin flap model was established and the animals were treated with HBO.Levels of neutrophil elastase（NE） and high mobility group B 1 （HMGB1） in the animals of all the groups were detected by ELLSA.Results NE levels of the IR 3-day group and the IR 5-d group were obviously higher than that of the sham group （P 〈0.01）,and following HBO treatment,NE levels of the 3-day and 5-day groups were significantly lower than that of the corresponding ischemia-reperfusion group（P 〈 0.05）.HMGB1 levels of the IR 3-day and 5-day groups were obviously higher than that of the sham group（P 〈 0.01）.HMGB1 level of the HBO 3-day group was significantly lower than that of the corresponding ischemia-reperfusion group （P 〈 0.01）,and HMGB1 level of the HBO 5-day group was lower than that of the corresponding ischemia-reperfusion group（P 〈 0.05）.Conclusions HBO treatment could obviously down-regulate the serum levels of NE and HMGB1,alleviate inflammatory reactions induced by ischemia-reperfusion lesion and increase survival rate of skin flaps in rats following skin flap transplantation.