PPARγ在人肺高、低转移涎腺腺样囊性癌细胞系中的表达及意义

Expression of peroxisome proliferator-activatived receptors gamma in SACC-LM and SACC-83 cells ZHANG

目的检测过氧化物酶体增殖物激活受体γ(PPARγ)在涎腺腺样囊性癌高、低转移细胞系中的表达,并探讨PPARγ是否与涎腺腺样囊性癌(SACC)肺转移转移相关。方法采用Western blot和逆转录-聚合酶链反应(RT-PCR)及实时定量PCR(Quantitative Real-time PCR)方法检测肺高转移性涎腺腺样囊性癌(SACC-LM)及肺低转移性涎腺腺样囊性癌(SACC-83)细胞系中PPARγ蛋白表达和PPARγmRNA的表达水平。所得实验数据采用SPSS 16.0统计软件进行t检验。结果 PPARγ蛋白及mRNA在SACC-LM细胞系及SACC-83细胞系中均有表达,与SACC-83细胞系相比,SACC-LM细胞系中PPARγ蛋白较高表达,并且SACC-LM细胞系中PPARγmRNA表达水平是SACC-83细胞中表达水平的4.346939倍(P<0.01)。结论 PPARγ在SACC肺高低转移细胞系中的差异表达,初步证实PPARγ在SACC远处转移方面有重要作用。

Objective To investment the change in expression of PPARγ in different cell lines of salivary adenoid cystic carcinoma（SACC）and whether PPARγ was related to the metastasis of salivary adenoid cystic carcinoma.Methods Reverse transcription-polymerase Chain reaction（RT-PCR）、Western blot and Quantitative Real-time PCR method were used respectively to detect mRNA and protein expression of PPARγin SACC-LM and SACC-83 cell lines.The data was analyzed with SPSS 16.0 software package for t-test. Results The expression of PPARγ mRNA and PPARγ in SACC-LM were obviously higher than that in SACC-83. Conclusion The changes in expression of PPARγmRNA and protein are related to the metastases of SACC. It is suggested that PPARγ may play an important role in the metastasis of adenoid cystic carcinoma.