CXCL12/CXCR4轴与胃癌细胞侵袭转移关系的研究

Relationship between CXCL12/CXCR4 axis and the invasion and metastasis in gastric cancer cells

目的研究趋化因子CXCL12对胃癌BGC-823细胞体外侵袭、转移能力的影响,进一步探讨CXCL12/CXCR4轴在胃癌侵袭、转移过程中的机制。方法采用Transwell法、real-time PCR法及免疫荧光法分别检测经不同浓度CXCL12处理后的胃癌BGC-823细胞侵袭能力的变化、MMP-2 mRNA表达量的变化、MMP-2蛋白表达的变化。结果与空白对照组比较,CXCL12不同浓度组穿膜细胞数和MMP-2 mRNA表达量均显著增多(P<0.001)。上述指标在CXCL12不同浓度组间比较,除200 ng/ml组与100 ng/ml组间相比,差异无统计学意义(P>0.05)外,其余各组间比较,差异均有统计学意义(P<0.001);免疫荧光结果显示,随着CXCL12浓度的升高,胃癌BGC-823细胞MMP-2蛋白表达增强。结论 CXCL12能增强胃癌BGC-823细胞的体外侵袭能力,并具有一定的量效关系,其促进胃癌侵袭、转移的机制可能与增强MMP-2的表达有相关性。

Objective To study the effects of chemokine CXCL12 in vitro invasion and metastasis of gastric cancer BGC-823 cells,and further explore the CXCL12 / CXCR4 axis possible mechanism of invasion and metastasis in gastric cancer. Methods Transwell method,real-time PCR assay and immunofluorescence were used to detect the changes of gastric cancer BGC-823 cells invasion ability,expression level of MMP-2 mRNA,expression of MMP-2 protein after treatment with different concentrations of CXCL12. Results Compared with the blank control group,the number of invasive cells and the expression of MMP-2 mRNA were significantly increased in different concentrations of CXCL12 groups（P 〈0. 001）. These indicators had significant statistical difference among different concentrations of CXCL12 groups（P 〈0. 001）,except no significant difference between 100 ng / ml group and 200 ng / ml group（ P 〉0. 05）. Immunofluorescence showed that with increasing concentrations of CXCL12,expression of MMP-2 protein in gastric cancer BGC-823 cells was enhanced. Conclusion CXCL12 can increase the invasion of gastric cancer BGC-823 cells in vitro,and has a certain dose-effect relationship,the mechanism of invasion and metastasis may be related to its enhancement of MMP-2expression.