猴脑严重缺血后选择性脑深低温灌注对神经功能的影响

Effect of Selective Cerebral Deep Hypothermic Perfusion on the Neurological Function of Rhesus Monkey after Severe Brain Ischemia

目的探讨严重脑缺血后行选择性脑深低温灌注对神经功能的影响.方法恒河猴9只,常温下阻断双侧颈总动脉10 min后行选择性脑深低温灌注60 min,实验动物自然复温.术后3 d以实验动物的中枢神经功能评分表为标准进行神经功能缺失评分,以后每周评分1次,共12周.结果术后3 d评价（13.56±1.81）,部分实验动物出现昏迷、嗜睡、跛行等情况,需要人工饲养生存,术后两周评价实验动物神经功能评分（16.11±1.76）,均可独立存活.与术前比较,实验动物术后3 d神经功能评分明显降低,于术后1周开始逐渐升高,术后3 d至术后6周与术前组两两比较差异有统计学意义（P〈0.05）,术后第7周-术后12周与术前比较差异无学意义（P〉0.05）.结论恒河猴严重脑缺血后行选择性脑深低温灌注,术后实验动物未出现严重脑缺血导致的偏瘫、昏迷、植物状态生存甚至死亡等情况,可以明显改善脑缺血的预后.

Objective To investigate the effect of selective cerebral deep hypothermic perfusion on the neurological function of rhesus monkey after severe brain ischemia. Methods Nine rhesus monkeys were given blocking bilateral common carotid arteries for 10 min at room temperature, and then keeping selective cerebral deep hypothermic perfusion for 60 mins, and then giving natural rewarming. After three days, the experimental animal central nervous function scale was used to evaluate the nerve function every week for 12 weeks. Results After three days evaluation（13.56 ± 1.81）, part of the experimental animals had such symptoms as coma, lethargy,lameness, etc., and needed to survive in captivity. After two weeks neurological evaluation（16.11 ± 1.76）,animals could survive independently. Compared with before operation, the neurological function in experimental animals was significantly lower 3 days after operation, and was gradually increased 1 week after operation. There were statistically significant differences in the neurological function score between 3 days to 6 weeks after operation and before operation（P〈0.05）, but no statistically significant difference between 7 to 12 weeks after operation and before operation（P〉0.05）. Conclusions Selective cerebral deep hypothermic perfusion after severe brain ischemia in Rhesus monkeys can significantly avoid severe hemiplegia, coma, vegetative state survival or death caused by cerebral ischemia, and can significantly improve the prognosis of cerebral ischemia.