3种胶质瘤细胞株(U87、U251及T98G)体外趋化组织细胞淋巴瘤细胞(U937)的实验研究

Experimental Study on The Chemotaxis of Three Kinds of Glioma Cell Line(U87,U251 and T98G) to Stiocytic Lymphoma Cell(U937) in Vitro

目的观察胶质瘤细胞株U87(人脑星形胶质母细胞瘤细胞)、U251(人神经胶质细胞瘤细胞)及T98G(人胶质母细胞瘤细胞)对U937(组织细胞淋巴瘤细胞)的趋化活性.方法体外传代培养U87、U251、T98G及U937细胞,应用Transwell法观察3种胶质瘤细胞株(U87、U251及T98G)对U937细胞的趋化活性.结果 Transwell小室下孔细胞离心浓缩后白血球计数板计数;3种胶质瘤细胞株对于U937细胞趋化活性明显高于正常对照组(P<0.05);U87对于U937细胞趋化活性明显高于U251及T98G(P<0.05);U251及T98G对于U937细胞趋化活性差异无统计学意义(P>0.05).结论 3种胶质瘤细胞株(U87、U251及T98G)可促进U937细胞的趋化迁移,U87趋化活性更为明显.

Objective To observe the chemotaxis of glioma cell line U87, U251 and T98 G to U937.Methods We Subcultured U87, U251, T98 G and U937 cells in vitro, used Transwell to observe the chemotaxis of three kinds of glioma cell line（U87, U251 and T98G） to U937. Results By Transwell method, we got the white blood cell count,compared with control group, the chemotaxis of three kinds of glioma cell line to U937 was apparently higher（P〈0.05）. The chemotaxis of U87 to U937 is apparently higher than U251 to T98G（P〈0.05）. The chemotaxis of U251 and T98 G to U937 had no statistical differences（P〉0.05）.Conclusion U87,U251 and T98 G can accelerate U937 cell migration, the chemotactic activity of U87 is more obvious.