两种短命植物HRD转录因子基因的克隆及序列分析

Cloning and Sequence Analysis of HRD Transcription Factors Gene from Two Kinds of Ephemeral Plants

以新疆短命植物线果芥(Conringia planisiliqua L.)和大蒜芥(Sisybrium altissimum L.)为试验材料,采用同源序列克隆的方法,分别从线果芥和大蒜芥c DNA中克隆得到了一条HRD转录因子基因,命名为Cp HRD和Sa HRD。通过生物信息学分析,Cp HRD和Sa HRD转录因子基因开放阅读框长564 bp,与山嵛菜(Eutrema salsugineum)、琴叶拟南芥(Arabidopsis lyrata)、荠菜(Capsella rubella)及拟南芥(Arabidopsis thaliana)核苷酸序列的同源性分别为88%、87%、87%和86%,编码187个氨基酸,基因编码蛋白分子量约为20 k D,二级结构包括2个β-折叠、5个a-螺旋和无规则卷曲,属含有信号肽的亲水性非跨膜稳定蛋白。亚细胞定位主要在细胞核中,系统进化树分析表明线果芥、大蒜芥和拟南芥属于同一种属,拥有1个典型的AP2/EREBP功能结构域,推测Cp HRD和Sa HRD基因属于AP2/EREBP转录因子家族EREBP亚组,可能参与植物ABA、干旱和低温等环境胁迫的应答过程,与植物的抗逆性有关。本研究为进一步深入探索HRD基因功能及其抗旱机制奠定了基础。研究同时也为短命植物种质资源的开发和利用发掘了新基因。

We isolated two transcription factor genes,named as Cp HRD and Sa HRD,from two kinds of ephemeral Conringia planisiliqua L. and Sisybrium altissimum L. by RT-PCR,respectively. By bioinformatics,both of Cp HRD and Sa HRD are possessing an intact complete open reading frame of 564 bp and sharing 88%,87%,87% and 86% of sequence identity with Eutrema salsugineum,Arabidopsis lyrata,Capsella rubella and Arabidopsis thaliana,respectively. The deduced polypeptide sequence of Cp HRD and Sa HRD are 187 amino acid residues with a predicted molecular weight of 20 k D with secondary protein structure of the polypeptide chains contain including 2 β-sheets,5 α-helixs and random coils,belonging to hydrophilicity and stable of transmembrane protein. By phylogenetic tree analysis,Cp HRD and Sa HRD mainly exist in the nucleuscontains,and have a typical AP2 / EREBP domain function structure,which may be involved in plant stresses response process of ABA,drought and low temperature. Our results can provide useful information about the molecular mechanism of HRD genetic mechanism of drought resistance,and will contribute a valuable resource for development and utilization of germplasm resources of ephemeral plant.