多酶共固定化反应体系的研究进展

Research Progress in Multi-enzyme Co-immobilization Reaction Systems

近年来,生物催化为化学、生物学和生物工程学等领域提供了一种绿色研究工具,其中多酶体系在这些领域中的应用越来越受到关注,其克服了以往单个酶不能满足催化需求的局限性,同时多酶共固定化在级联反应过程中,可增加酶周围的反应物浓度,并将不同酶的催化特性结合起来,能排除干扰因素,从而提高酶的整体催化效率。对多酶共固定化反应体系的研究进展进行了综述,包括多酶反应体系的类别、共固定化技术的特点以及相关应用,并对共固定化多酶反应体系进行了展望。

In recent years,biocatalysis has started to provide an important green study tool in chemistry、biology、Bioengineering and so on,currently,the ideal of using multi-enzymatic systems for these fields becomes increasingly attractive,it overcomes this limitation that many situations where a single enzyme cannot completely catalyze reactions,meanwhile,in the cascade reactions,it can cause the enzyme to act under a higher substrate concentration and combine different enzyme catalytic properties,also can eliminate interference factors by using the co-immobilized enzymes,therefore the whole catalytic efficiency may be improved. The research progress in Multi-enzyme Co-immobilization reaction systems was reviewed in three aspects including the classification of multi-enzyme reaction systems、the characteristics and applications of co-immobilization technology. In addition,Multi-enzyme Co- immobilization reaction systems were also predicted.