枸杞对小鼠辐射损伤后造血系统修复的促进作用

Promoting effect of Lycium chinense Mill on hematopoietic reconstruction in mice after radiation injury

目的探讨枸杞在小鼠辐射损伤后造血系统修复过程中的作用。方法小鼠随机分为3组,正常对照组不做任何处理,喂饲正常饲料;另外2组,给予4.5 Gy照射后,喂饲正常饲料或添加枸杞的饲料,1个月后,处死小鼠,应用流式细胞仪分析外周血、脾脏、胸腺及骨髓中各种血细胞。结果枸杞饲料组小鼠,外周血细胞总数及B细胞增多,骨髓细胞总数减少,骨髓前体B细胞及未成熟B细胞增多,造血干、祖细胞均显著减少。结论枸杞促进辐射损伤后造血干细胞动员及分化,加速造血系统的恢复。

Objective To investigate the effect of Lycium chinense Mill on hematopoietic reconstruction in mice after radiation injury. Method Female C57 BL /6J mice were grouped to 3 groups at random： the control group,with normal food and no treatment,and the other 2 groups,treated with 4. 5 Gy irradiation,with normal food or food added with Lycium chinense Mill. After 1 month,the mice were sacrificed and analyzed the peripheral blood,spleen,thymus and bone marrow cells by flow cytometer. Results In the mice fed with Lycium chinense Mill,the percentage of B cells was increased in the peripheral blood,the pre-B and immature B cells were increased,too,and the percentage of hematopoietic stem / progenitor cells was decreased. Conclusion Lycium chinense Mill can promote the mobilization and differentiation of hematopoietic stem / progenitor cells,and accelerate the hematopoietic reconstruction after irradiation injury.