Slug和E-cadherin在肾透明细胞癌组织中的表达及临床意义

Expression of Slug and E- cadherin in clear cell renal cell carcinoma and their significance

目的:研究Slug和E-cadherin在肾透明细胞癌(clear cell renal cell carcinoma,ccRCC)组织中的表达及其临床意义。方法:采用免疫组化SP法检测83例ccRCC组织和40例癌旁组织中Slug和E-cadherin蛋白的表达。采用Real-time PCR检测20例ccRCC及癌旁新鲜组织中Slug和E-cadherin mRNA的表达。分析二者蛋白和mRNA表达的相关性及Slug和E-cadherin蛋白表达与临床病理参数及预后的相关性。结果:Slug蛋白和mRNA在ccRCC组织中的表达明显高于癌旁组织。E-cadherin蛋白和mRNA在ccRCC组织中的表达明显低于癌旁组织(P<0.05)。ccRCC组织中Slug和E-cadherin蛋白和mRNA表达均具有显著负相关性(P<0.05)。Slug蛋白的表达与肿瘤大小、复发、TNM分期、淋巴结转移及血管侵袭密切相关(P<0.05);E-cadherin蛋白的表达与性别、肿瘤大小、TNM分期、淋巴结转移及血管侵袭密切相关(P<0.05)。Slug蛋白高表达的患者预后明显差于低表达的患者(P<0.05);而E-cadherin蛋白高表达的患者预后明显优于低表达的患者(P<0.05)。COX多因素回归分析显示Slug和E-cadherin蛋白表达状态是影响ccRCC患者预后的独立因素。结论:Slug和E-cadherin在ccRCC发生发展中发挥重要作用,可作为ccRCC预后判断的生物标志物。

Objective：To investigate the expression of Slug and E - cadherin in clear cell renal cell carcinoma （ccRCC）and its clinical significance. Methods：To detect the protein expression of Slug and E - cadherin in 83 ca-ses of ccRCC tissues and 40 cases of tumor - adjacent tissues by immunohistochemistry,detect the mRNA expression of Slug and E - cadherin in 20 cases of ccRCC and tumor - adjacent fresh tissues by Real - time PCR,analyze the relationship between Slug and E - cadherin protein and mRNA expression and the relationship between the protein expression of Slug,E - cadherin and clinicopathological parameters and prognosis. Results：The expression of Slug protein and mRNA in ccRCC tissue were significantly higher than that in the tumor - adjacent tissues,while the expression of E - cadherin protein and mRNA in ccRCC tissue were significantly lower than that in the tumor - adjacent tissues （ P 〈0. 05）. There was significant negative correlation between Slug and E - cadherin protein and mRNA expression in ccRCC tissue（ P 〈 0. 05）. The expression of Slug protein was closely related with tumor size,TNM stages, lymph node metastasis,vascular invasion and recurrence（ P 〈 0. 05）. The expression of E - cadherin protein was closely related with gender,tumor size,TNM stages,lymph node metastasis and vascular invasion（ P 〈 0. 05）. The prognosis of patients with high expression of Slug protein was significantly worse than the patients with low expression （P 〈0. 05）,while the prognosis of patients with low expression of E - cadherin protein was significantly worse than the patients with high expression of E - cadherin（P 〈 0. 05）. Multivariate COX regression analysis showed that Slug and E - cadherin protein expression were the independent prognostic factors for patients with ccRCC. Conclusion： Slug and E - cadherin play an important role in the development of ccRCC,which can be used as biomarkers in judging the prognosis of ccRCC.