摘要
采用实时定量逆转录PCR方法（qRT—PCR）检测30例前列腺癌、40例前列腺增生以及50例正常人血清中miRNA141和miRNA375的表达情况，研究三者间的表达差异。血清miRNA141在前列腺癌组、增生组的表达量分别为正常人组的13．4±7．2、1．5±1．7倍，差异有统计学意义（P〈0．05）；血清miRNA375在前列腺癌组、增生组的表达量分别为正常人组的28．4±10．3、1．9±2．1倍，差异有统计学意义（P〈0．05）。
To explore the diagnostic values of miRNA141 and miRNA375 for prostate cancer. The expressions of miRNA141 and miRNA375 in serum samples fi＇om 30 prostate cancer （PCA） patients,40 benign prostatic hyperplasia patients and 50 normal controls were detected by quantitative reverse transcription-polymerase chain reaction （qRT-PCR）. The expression of miRNA141 in tissue samples from prostate cancer was 13. d ± 7.2 folds of that from benign prostatic hyperplasia while the expression of miRNA375 in tissue samples from prostate cancer was 28.4 ± 10. 3 folds of that from benign prostatic hyperplasia. The differential expression of miRNA141 and miRNA375 in sera of prostate cancer suggested that miRNA141 and miRNA375 mizht be ootential tumor markers in clinical practice.
出处
《中华全科医师杂志》
2015年第3期228-230,共3页
Chinese Journal of General Practitioners
基金
温州市科技计划项目（Y20120160）
关键词
前列腺肿瘤
实验室技术和方法
Prostatic neoplasms
Laboratm3, techniques and procedures
