基于旋光法的原料乳中乳糖掺伪鉴别技术研究

Study on the technology of identify the lactose adulteration in raw milk based on polarimetric method

旨在建立一种简便、高效的原料乳乳糖掺伪鉴别方法。以三氯乙酸为沉淀剂进行前处理,借助旋光仪,在589.30-589.44nm下,测定标准乳糖溶液的旋光度,绘制标准曲线,建立了一种基于标准曲线的原料乳乳糖掺伪定量鉴别法。所得回归方程的线性相关系数R^2=0.9993,平均回收率达99.0%,相对标准偏差（RSD）为0.67%。与莱因-埃农氏法进行比对实验,结果无差异,但更快捷,成本更低。研究结果提示,本文所建方法实用、可靠。

This paper was aimed to set a simple and highly-efficient method for lactose adulteration detection in raw milk. After pretreatment using trichloroacetic acid as precipitant,at 589.30-589.44nm,the optical rotation was measured by polarimeter to create standard curve about standard lactose solution. So as to establish a polarimetric method for quantitative detection of the lactose adulteration in raw milk based on standard curve. The correlation coefficent（R^2） of this regression equation acquired in our study was equivalent to 0.9993;the average recovery rate was 99.0%;the relative standard deviation was 0.67%. In the comparison test,the results showed no significant difference between the Laing Henon＇method and the one established in this study, but the latter was simpler and cheaper than the former. It was suggested that such method was practical and reliable.