响应面法优化山杏核壳总黄酮提取工艺及其抗氧化性的研究

Study on optimization of extraction of total flavonoids from shell of wild apricot by response surface methodology and its antioxidant activity

以山杏核壳为原料,采用闪式提取技术对山杏核壳中的总黄酮进行提取,在单因素实验的基础上,采用响应面分析法对闪提工艺进行优化,并通过体外抗氧化实验,测定其抗氧化能力。最终得到较优提取工艺参数为:乙醇溶液体积分数62%,闪提时间4min,液料比13∶1,温度53℃。此条件下山杏核壳总黄酮得率为12.93mg/g。抗氧化实验表明,山杏核壳总黄酮有很好的还原性,对羟基自由基、DPPH·和超氧阴离子都有较好的清除能力,具有良好的抗氧化性。

The shell of wild apricot were used as the raw material to extract total fiavonoids by flash-extraction, based on single factor experiments ,extraction condition was optimized by response surface methodology. The results showed that the optimum conditions of extraction of the total flavonoids from shell of wild apricot were as follows：62% ethanol concentration,4min extraction time, 13：1 liquid/material ratio and 53℃ extraction temperature. Under this condition,the maximal content of total flavonoids was up to 12.93mg/g. Results of antioxidation showed that the total flavonoids from shell of wild apricot had a good ability to scavenge hydroxyl radicals, DPPH, superoxide anion, it had good antioxidant activity.