BMSCs复合PHBV原位修复兔鼻中隔软骨缺损的实验研究

BMSCs-PHBV in the In-situ Remediation of Nasal Septal Defect in Rabbit

目的探索BMSCs复合PHBV原位修复兔鼻中隔软骨缺损的可行性。方法建立兔鼻中隔软骨缺损模型。将36只健康新西兰大白兔随机分成3组。实验组:软骨缺损处植入BMSCs-PHBV复合物进行修复;对照组:软骨缺损处植入单纯PHBV支架材料进行修复。空白组:单纯取出鼻中隔软骨,不予修复。分别于16、24周取材,行大体观察及组织学检测。结果大体观察显示,实验组新生软骨样组织将鼻中隔软骨缺损区填充修复,与周围软骨组织未见明显分界;对照组新生软骨薄,与周围组织分界明显;空白组鼻中隔软骨缺损区未生成软骨组织,缺损部位及其周围正常软骨组织被纤维结缔组织充填覆盖。组织学检测显示,实验组构建的软骨组织结构致密,基质及Ⅱ型胶原显色程度均明显强于对照组构建的软骨。结论 BMSCs-PHBV复合物能有效修复兔鼻中隔软骨缺损。

Objective To explore the feasibility of the in-situ remediation of nasal septal defect in rabbit by BMSCs combing PHBV. Methods The nasal septal defects were surgically created in the 36 rabbits. The rabbits were randomly divided into 3 groups according to the repair materials. The experimental group： BMSCs-PHBV complex; the control group：only PHBV; the blank group： nothing. The specimen in each group were harvested for gross observation, HE and immunohistochemistry staining 16 and 24 weeks after operation. Results The defects were repaired by the hyline-like cartilage tissue without obvious boundary in the experimental group while the defects were repaired by thinner cartilage tissue with obvious boundary in the control group. And the defects were repaired by the fibrous tissues in the blank group.Histology and immunohistochemistry demonstrated that the cartilage formed have more compact tissue structure in the experimental group. The cartilage matrix staining of cartilage formed in the experimental group were stronger than that of cartilage formed in the control group. Conclusion BMSCs-PHBV complex could repair defects of nasal septal cartilage in rabbits effectively.