中药复方糖痹康对糖尿病大鼠坐骨神经BDNF蛋白及BDNFmRNA表达的影响

Effects of Chinese herbal compound Tangbikang on expression of BDNF protein and BDNFmRNA in sciatic nerve in diabetic rats

目的:观察中药复方糖痹康对糖尿病大鼠坐骨神经脑源性神经生长因子(Brain-derived neurotrophic factor,BDNF)蛋白及BDNFmRNA表达的影响。方法:采用高脂饲料和小剂量链脲佐菌素(streptozotocin,STZ)诱发2型糖尿病大鼠动物模型,用不同剂量的糖痹康灌胃并与甲钴胺对照,16周后取大鼠一侧坐骨神经,用免疫组化法检测坐骨神经中BDNF蛋白的表达及采用RTPCR方法检测坐骨神经组织中BDNF mRNA的表达。结果:与正常组比较,模型组BDNF蛋白表达显著降低及BDNF mRNA表达显著降低;糖痹康组与模型组比较,能升高BDNF蛋白及BDNF mRNA表达。结论:中药复方糖痹康能够促进糖尿病周围神经病变大鼠坐骨神经BDNF mRNA的转录和蛋白的表达。

Objective： To explore the effect of Chinese herbal compound Tangbikang on the expressions of BDNF protein and BDNF mRNA of diabetic rats＇ sciatic nerve. Methods： The experimental diabetes mellitus rats model were induced by feeding high fat forage and injection streptozotocin. Tangbikang groups were given Tangbikang with different dose,rats unilaterals sciatic nerve were cutting-up after 16 weeks,immunohistochemical method was used to detect the expressions of BDNF protein of sciatic nerve,and the expressions of BDNFmRNA were detected by semi-quantitative RTPCR,and compared with mecobalamine group. Results： The expressions of BDNF protein and BDNFmRNA of model group were significantly decreased compared with normal group,Tangbikang group significantly increased the expressions of BDNfprotein and BDNFmRNA. Conclusion： Chinese herbal compound Tangbikang could promote the expressions of BDNFprotein and BDNFmRNA of diabetic peripheral neuropathy rats＇ sciatic nerve