以超声辅助浊点萃取法提取竹叶中的叶绿素

Chlorophyll in Bamboo Leaves Extracted via Ultrasound-assisted Cloud Point Extraction

以绿色环保的浊点萃取法,并以超声辅助提取分离了竹叶中的叶绿素,对试验条件进行了优化,其萃取效果优于同等试验条件下的乙醇提取法。通过对表面活性剂Genapol X-080的质量浓度、液固比、超声时间、p H进行单因素试验,再在最优条件范围内,正交试验得出最佳试验条件：表面活性剂的质量浓度为3 g/100 m L,液固比为25 m L/g,超声时间为20 min,p H 6~7。结果表明,利用浊点法提取竹叶中叶绿素的方法极具研究价值与发展前景。

In this work,cloud point extraction and ultrasound-assisted extraction was applied to extract chlorophyll from bamboo.Effects of the extract conditions were investigated by orthogonal experiments on the basis of single-factor tests in which the effects of concentration of surfactant Genapol X-080,ratio of material to liquid,extraction times and pH were discussed. The surfactant extraction was compared with conventional solvent： ethanol,the results shows that the extraction effect was better than the same experiment ethanol. The optimized extraction conditions were obtained： concentration of 3 g /100 mL surfactant,liquid to solid ratio25,ultrasonic time of 20 min,pH is 6 ~ 7.