HPLC测定相思子叶中滨蓟黄素含量

Determination of Cirsimaritin in the Leaves of Abrus Precatorius L. by HPLC

目的建立HPLC测定相思子叶中滨蓟黄素含量的方法。方法色谱条件为色谱柱Hypersil BDS C18（4.6mm×150mm,5μm）,流动相为甲醇（A）-0.2%甲酸水溶液（B）,二元梯度梯度洗脱,检测波长为330nm,流速为1.0m L·min^-1。结果滨蓟黄素在0.062~0.31μg与其峰面积呈良好的线性关系,其线性回归方程为Y=241.91X＋19.264,r=0.9993,平均加样回收率为99.37%（RSD=1.37%）。结论该方法简便准确、分离度好、专属性强,可用于相思子叶中滨蓟黄素的质量控制。

OBJECTIVE To establish a HPLC method for the determination of cirsimaritin in the leaves of Abrus precatorius L. METHODS Hypersil BDS C18column（ 4. 6mm × 150 mm,5μm） was used,and the mobile phase was consisted of methanol and water using a gradient elution. The detection wavelength was at 330 nm,the flow rate was1. 0m L·min^- 1. RESULTS Cirsimaritin showed a good linear relationship from 0. 062 to 0. 31μg（ r = 0. 9993）,and the regression equation was Y = 241. 91 X ＋ 19. 264（ r = 0. 9993）. The average recovery was 99. 37% with RSD1. 37%. CONCLUSION The HPLC method is simply and accurate with a good specificity. It can be used to quality control of cirsimaritin in the leaves of Abrus precatorius L.