摘要
目的：建立HPLC法测定非离子型表面活性剂泡囊中蛇床子素的含量。方法：采用Luna C18（250 mm×4.6mm,5μm）色谱柱,以甲醇-水（85∶15）为流动相,流速1.0 ml/min,320 nm处检测。结果：蛇床子素在浓度0.4~50μg/ml范围内线性关系良好,回归方程为A=93 807 C＋18 201（r=0.999 9）,平均回收率为98.09%,RSD为0.03%。结论：本法快速、准确,结果可靠,重现性好,适于蛇床子素制剂的质量控制。
Objective： To establish an HPLC method for the determination of osthole in nonionic surfactant vesicles. Methods： A Luna C18（ 250 mm × 4. 6 mm,5 μm） column was used as stationary phase,and methanol- water（ 85∶ 15） as mobile phase with a flow rate of 1. 0 ml / min. The wavelength was set at 320 nm. Results： The linear range of osthole was 0. 4 ~ 50 μg / ml,the regression equation was expressed by A = 93 807 C ＋ 18 201（ r = 0. 999 9）,the average recovery was 98. 09%,and RSD 0. 03%. Conclusion： this method is rapid,accurate,reliable,reproducible,suitable for the quality control of osthole preparation.
出处
《天津药学》
2015年第1期19-21,共3页
Tianjin Pharmacy
