心肌缺血对大鼠肾皮质利钠肽受体表达的影响

Effect of myocardial ischemia on the expression of natriuretic peptide receptor in renal cortex of rats

目的探讨大鼠肾皮质利钠肽受体A(NPR-A)和受体C(NPR-C)的表达在心肌缺血后不同时程的动态变化。方法大鼠分为空白对照组、假手术组和模型组。分别于复制心肌缺血模型后的第3、7、14、18、28天取材,用Western blotting方法检测肾皮质内NPR-A和NPR-C的表达。结果在上述时间点上,肾皮质NPR-A的表达显著性降低(P<0.01),NPR-C表达呈现由缺血后第3天到缺血后第18天由低到高的波动变化,到缺血后第28天逐渐恢复至空白对照组水平。结论心肌缺血后,动物机体钠和水的滞留与NPR-A表达的降低以及NPR-C表达的升高密切相关。

Objetcive To investigate the changes of the expression levels of natriuretic peptide receptor A （ NPRA-） and C （NPR-C） in renal cortex after myocardial ischemia （MI） in rats.Methods All the rats were randomly divided into blank control group , sham-operation group and myocardial ischemia group .After MI models were established , the rats were sacrificed,and the expression levels of NPR-A and NPR-C in renal cortex were detected by Western blotting on day 3,7,14, 18,28.Results The expression levels of NPR-A in renal cortex were significantly decreased in different time points mentioned above （ P〈0.01）,however,the expression levels of NPR-C were increased from day 3 to day 18,then,which gradually recovered to the levels in blank control group on day 28.Conclusion After myocardial ischemia ,the detention of sodium and water in the kidney of rats is closely correlated to the decrease of expression levels of NPR -A and the increase of expression levels of NPR-C.