VEGF、P53及C-erbB2蛋白在卵巢上皮性肿瘤中的表达及临床意义

Expression and clinical significance of VEGF,P53,C-erbB2 protein in epithelial ovarian neoplasms

目的观察卵巢上皮性肿瘤组织中血管内皮生长因子(VEGF)、抑癌基因P53及人表皮生长因子受体2(C-erbB2)蛋白的表达情况,探讨三者在卵巢肿瘤发生发展中的作用。方法采用免疫组化SP法检测40例正常卵巢、45例良性、30例交界性、65例卵巢上皮癌中VEGF、P53及C-erbB2蛋白的表达。结果正常卵巢、良性、交界性、卵巢上皮癌中VEGF蛋白的表达率分别为17.50%(7/40),24.44%(11/45),63.33%(19/30)和73.85%(48/65),差异有统计学意义(P<0.05)。卵巢癌组VEGF蛋白的表达与肿瘤的临床分期差异有统计学意义(P<0.05),但与肿瘤的病理类型及组织分化程度差异无统计学意义(P>0.05);正常卵巢、良性、交界性、卵巢上皮癌中P53蛋白的表达率分别为20.00%(8/40)、24.44%(11/45)、56.67%(17/30)、56.92%(37/65),4组中C-erbB2蛋白的表达率分别为15.00%(6/40)、15.56%(7/45)、50.00%(15/30)、67.69%(44/65),4组比较,差异有统计学意义(P<0.05)。CerbB2蛋白表达与肿瘤的临床分期差异有统计学意义(P<0.05)。VEGF蛋白在卵巢上皮癌中的表达与P53及CerbB2蛋白表达呈正相关(r值分别为0.44和0.23,两者均P<0.05),共同促进卵巢癌发生发展。结论 VEGF、P53及C-erbB2蛋白在卵巢癌发生、发展中起重要作用,P53及C-erbB2在癌基因方面促进血管生成因子VEGF的发生,共同促进卵巢肿瘤的发生发展。三种蛋白的联合检测对卵巢肿瘤的诊断、治疗及预后评价有一定的指导意义。

Objective To investigate the expression of vascular endothelial growth factor（ VEGF）,P53 and human epidermal growth factor receptor 2（ C-erb B2） protein in epithelial ovarian tumors,and to explore their roles in the pathogenesis and development of epithelial ovarian tumors. Methods The expression rates of VEGF,P53 and C-erb B2 protein in 40 cases of normal ovaries,45 cases of benign ovarian tumors,30 cases of borderline ovarian tumors and 65 cases of epithelial ovarian tumors were detected by immunohistochemistry（ SP）. Results The positive expression rates of VEGF protein were 17. 50%（ 7 /40）,24. 44%（ 11 /45）,63. 33%（ 19 /30）,73. 85%（ 48 /65） in normal ovaries,ovarian benign tumors,ovarian borderline tumors,ovarian epithelial tumors,respectively,there were significant differences among them（ P〈0. 05）. The expression levels of VEGF protein were correlated to clinical staging in ovarian cancer group（ P〈0. 05）,however,which were not related with pathological type and histodifferentiation degree of ovarian cancer（ P〈0. 05）. The positive expression rates of P53 protein were 20. 00%（ 8 /40）,24. 44%（ 11 /45）,56. 67%（ 17 /30）,56. 92%（ 37 /65） in normal ovaries,benign ovarian tumors,ovarian borderline tumors,ovarian epithelial tumors,respectrively,however,the positive expression rates of C-erb B2 protein were 15. 00%（ 6 /40）,15. 56%（ 7 /45）,50. 00%（ 15 /30）,67. 69%（ 44 /65） in the four kinds of tissues,respectively,there were significant differences among the 4 groups（ P〈0. 05）. The expression levels of C-erb B2 protein were correlated to clinical staging of ovarian cancer（ P〈0. 05）. The expression levels of VEGF were positively correlated to those of P53 and C-cerb B2 in ovarian epithelial tumors（ r = 0. 44,r = 0. 23,P〈0. 05）,which promoted the pathogenesis and development of ovarian cancer together. Conclusion The VEGF,P53 and C-erb B2 play an important role in the pathogenesis and development of ovarian cancer,and P53 and C-erb B2 increase the expression of VEGF in oncogenes,which promote the pathogenesis and development of ovarian cancer together. The combined detection of the three kinds of proteins is valuable in the diagnosis,treatment and prognostic evaluation of ovarian neoplasms.