三峡库区灰蜗牛微孢子虫检出度的研究

Study on the detection of microsporidia from Fruticicola ravida in the Three Gorges Reservoir area

为了防范野生灰蜗牛微孢子虫对家蚕的交叉感染风险和研发新型农业害虫生物防治剂,试验采用不同浓度梯度的家蚕微孢子虫模拟感染灰蜗牛,进行了灰蜗牛体内微孢子虫检出度的研究。结果表明：当样本中微孢子虫浓度低于8×102个/m L时,镜检到微孢子虫的检出度为0;当样品中微孢子虫浓度等于或大于8×103个/m L时,镜检到微孢子虫的检出度为1;当样品中微孢子虫浓度介于8×102--8×103个/m L之间时,检出微孢子虫具有随机性。说明当采用人工镜检的方法从桑园附近的野生灰蜗牛体内检测不到微粒子微孢子虫时,也需要加强其体内携带的微量微孢子虫对家蚕交叉感染风险的防控工作。

To prevent the risk of cross- infection of microsporidia from wild Fruticicola ravida for Bombyx mori,and develop new bio- control preparations for agricultural pests. The study on the detectability of microsporidia from Fruticicola ravida was conducted using different concentration gradients of Nosema bombycis to mock- infect Fruticicola ravida. The results showed that the detectability of microscopic examination of the spores was 0 when the concentrations of Nosema bombycis in the samples were lower than 8 × 102 spores per m L; the detectability of microscopic examination of the spores was 1 when the concentrations of Nosema bombycis in the samples were equal to or greater than 8 × 103 spores per m L; the detectability of microscopic examination of the spore was random when the concentrations of Nosema bombycis in the samples were between 8 × 102 and 8 × 103 spores per m L. The results indicate that the prevention and control work of the cross- infection of microsporidia in Fruticicola ravida for Bombyx mori should also be strengthened when the Nosema bombycis from Fruticicola ravida near the mulberry park cannot be detected using artificial microscopic examination.