摘要
腐霉猝倒和根腐病引起大豆出苗率严重减少,因此造成减产。有报道认为大豆品种Archer对由腐霉菌引起的苗期病害具有抗性,并描述了与之相关的Rps1k基因对疫霉菌具有抗逆性。为了描述大豆品种Archer抗腐霉猝倒病的遗传特性,并且确定该抗性是否与Rps1k基因相关,Archer和Hutcheson（感病亲本）进行杂交,F2：4体（子二代）产生。
Pythium damping-off and root rot of soybean[Glycine max （L.） Merr.] causes poor stands and consequently reduces yields. Resistance to seedling diseases caused by Pythium spp. was reported in the soybean cultivar Archer and was dscribed to be associated with the Rpslk gene for resistance to Phytophthora sojae. To characterize the inheritance of Py- thium damping-off resistance in Archer and to determine if this resistance is linked to Rpslk, the cross of Archer ＇Hutcheson＇ （susceptible parent） was made and F~：~ lines generated. Parents and F2：4 lines were screened with Pythium aphanidermatum using a hypocotyl inoculation technique. Five days after inoculation, plant survival was scored. The F2：4 lines and parents were also inoculated with race 7 of P. sojae to assess the association of resistance to Pythium spp. with the Rpslk gene. Data from the F2：4 lines fit the model for a single dominant gene in Archer that confers resistance to P. aphanidermatum. The genetic linkage test showed that resistance to P. aphanidermatum was independent of the Rpslk gene in Archer. To identify the genomic location of the Pythium resistance gene, F2：4 lines were screened with simple se- quence repeat （SSR）markers from all the soybean molecular linkage groups （MLGs）. Two SSR markers, Satt510 and Satt114 on MLG F, which were polymorphic between the parents and the resistant and susceptible bulks, were shown to be associated with Pythium resistance. The Pythium resistance gene, named Rpal, was located 10.6 cM from Satt510 and 26.6 cM from Satt114.
出处
《大豆科技》
2015年第1期39-39,共1页
Soybean Science & Technology
