摘要
目的研究单纯性先天性心脏病(CHD)患者血浆miR-324-5p表达变化,评价其在CHD预后和风险分级等方面的临床应用价值。方法选取2012年1月至2013年1月沈阳军区总医院先天性心脏病内科收治的CHD患儿76例为CHD组,另选取13例健康者血清标本为对照组,应用实时荧光定量聚合酶链反应(PCR)检测血浆中miR-324-5p的表达变化,通过Kalpan-Meier生存分析、Cox比例风险模型等综合评价血浆miR-324-5p在CHD预后和风险分级等方面的临床应用价值。结果与对照组相比,单纯性CHD患者外周血浆miR-324-5p表达升高,差异有统计学意义(P<0.05);Kaplan-Meier生存分析表明,miR-324-5p表达水平较高组患者复发风险及死亡概率更高(P<0.05);Cox比例风险模型分析表明,在单因素分析中,miR-324-5p表达水平和体质量指数(BMI)是CHD的发病危险因子(P<0.05),风险比(HR)分别为1.79和2.17;在多因素分析中,只有BMI是CHD的发病危险因子(P<0.05),HR为1.38,HR 95%置信区间(CI)为(1.12,1.79)。结论血浆miR-324-5p作为CHD诊断的生物学标志物具有一定的临床应用价值,但还需要与其他指标联合应用。
Objective To investigate the change of plasma miR-324-5p expression in the patients with simple congenital heart disease（CHD）and to evaluate its clinical application value in the prognosis and the risk classification of CHD.Methods 76 children patients with CHD and 13 healthy controls were detected the plasma expression levels of miR-324-5p by the real-time PCR.The clinical application value of plasma miR-324-5p in the prognosis and the risk classification of CHD was comprehensively evaluated by adopting the Kalpan-Meier survival analysis,Cox proportional hazard model.Results The peripheral plasma miR-324-5p expression level in the simple CHD group was increased compared with the control group,the difference had statistical significance（P〈0.05）;the Kalpan-Meier survival analysis indicated that the higher miR-324-5p expression group had much higher recurrent risk and much higher death probability（P〈0.05）;the Cox proportional hazard model indicated that in the univariate analysis,miR-324-5p and BMI were the risk factors of CHD（P〈0.05）.The hazard ratio（HR）was 1.79 and 2.17 respectively.The multivariate analysis demonstrated that only BMI was the risk factor of CHD（P〈0.05）,HR was 1.38,95%CI（1.12,1.79）.Conclusion Plasma miR-324-5p as a biomarker of CHD diagnosis could have certain clinical application value,but should be combined application with other indicators.
出处
《检验医学与临床》
CAS
2015年第5期603-605,共3页
Laboratory Medicine and Clinic
