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DNA甲基化对杆状病毒极早期和滞早期启动子转录活性的影响 被引量：1

The Effect of DNA Methylation on the Transcriptional Activity of Some Immediate and Delay Early Gene Promoters of Baculovirus
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摘要 利用体外DNA甲基化酶和荧光素酶报告系统分析了DNA甲基化对杆状病毒苜蓿银纹夜蛾核型多角体病毒(Autographa californica multicapsid nucleopolyhedrovirus,AcMNPV)的8个极早期和滞早期基因启动子表达活性的影响.研究结果表明,病毒极早期基因ie1和ie0启动子的活性受到甲基化修饰的强烈抑制,pe38启动子活性受到部分抑制;同时滞早期启动子p35、DNApol、lef1、lef3和lef8的启动子则在甲基化处理后得到一定程度的激活.转染的同时用AcMNPV感染细胞,在多数启动子中并没有改变甲基化处理对它们表达活性的影响,但使其影响程度有所减弱.在另一些启动子(pe38,p35,lef1)中,AcMNPV的感染使甲基化的作用基本消失.这些结果表明DNA甲基化对一些杆状病毒启动子的转录活性有一定的调控作用. Abstract： In vitro DNA methylation and luciferase reporter system were used to analyze the effect of DNA methylation on the transcriptional activity of eight early promoters of baculovirus AeMNPV （Autographa californica multicapsid nucleopolyhedrovirus）. The result showed that the activity of virus immediate early（i, e. ） promoters ie0 and iel was greatly inhibited by DNA methylation, whereas the activity of another i. e. promoter, pc38 promoter was partially inhibitedafter methylation. Meanwhile, the activity of virus delayed early promoters, including promoters of 1-5, DNApol, lefl, lef3 and lefS, was significantly increased after methylation. AcMNPV infection did not altered the effeet of methylation in most promoters, only narrowed the difference between methylated and un-methylated promoters. However, in promoters of pc38, P35 and lefl, no significant difference were observed between methylated and un-methylated plasmid in the presence of AcMNPV infection. These results indicated that methylation played a role at least in the expression of some baculovirus genes.
作者 张琦 徐博文 王鑫 尹隽 钟江
机构地区 复旦大学生命科学学院微生物学与微生物工程系
出处 《复旦学报（自然科学版）》 CAS CSCD 北大核心 2015年第1期16-21,共6页 Journal of Fudan University：Natural Science
基金 国家自然科学基金资助项目(31170143)
关键词 杆状病毒 启动子 DNA甲基化 基因表达 baculovirus promoter DNA methylation gene expression
分类号 Q786 [生物学—分子生物学]
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参考文献17

  • 1Rohrmann G K Baculovirus Molecular Biology[M]. 2rd Edition Elnternet. Bethesda (MD): National Center for Bioteehnology Information (US), 2013.
  • 2Fujita R, Ohtsuka D, Sahara K, et al. An HDAC inhibitor increases AcMNPV gene expression in marrmlaliah eells[J]. Arch girol, 2010,155(4) : 577-581.
  • 3唐梦瑶,钟江.杆状病毒AcMNPVie0/ie1启动子区域甲基化对自身调控作用的影响[J].复旦学报（自然科学版）,2013,52(6):717-723. 被引量：3
  • 4Stewart T M, Huijskens I, Willis L G, et al. The Autographa californica multiple nucleopolyhedrovirus ieO-iel gene complex is essential for wild-type virus replication, but either IE0 or IE1 can support virusgrowth[J]. J Virol, 2005,79(8)= 4619-4629.
  • 5Milks M L, Washburn J O, Willis L G, et al. Deletion of pe38 attenuates AcMNPV genome replication, budded virus production, and virulence in Heliothis virescens[J]. Virology, 2003,310(2): 224-234.
  • 6Clem R J, Fechheimer M, Miller L K. Prevention of apoptosis by a baculovirus gene during infection of insect cells[J]. Science. 1991,254(5036) : 1388-1390.
  • 7Tomalski M D, Wu J G, Miller L K. The location, sequence, transcription, and regulation of a baculovirus DNA polymerase gene[J]. Virology, 1988,167(2): 591-600.
  • 8Passarelli A L, Miller L K. Identification and characterization of lef-1, a baculovirus gene involved in late and very late gene expression[J]. J Virol, 1993,67(6): 3481-3488.
  • 9Mikhailov V S, Rohrmann G F. Baculovirus replication factor LEF-1 is a DNA primase[J]. J Virol, 2002,76(5) : 2287-2297.
  • 10Li Y,Passarelli A L, Miller L K. Identification, sequence, and transcriptional mapping of lef-3, a baculovirus gene involved in late and very late gene expression[J]. J Virol, 1993,67(9): 5260-5268.

二级参考文献15

  • 1Huijskens Ilse, Li L, Willis L G, et al. Role of AcMNPV IE0 in baeulovirus very late gene activation [J]. Virology, 2004, 323(1): 120-130.
  • 2Kovacs G R, Guarino L A, Summers M D, et al. Novel regulatory properties of the IE1 and IE0 transactivators encoded by the baculovirus Autographa californica multicapsid nuclear polyhedrosis [J]. J Virol, 1991, 65(10): 5281-5288.
  • 3Chisholm G E, Henner D J. Multiple early transcripts and splicing of the Autographa californica nuclear polyhedrosis virus IE-1 gene [J]. J Virol, 1988, 62(9): 3193 -3200.
  • 4Lu L, Du Q, Chejanovsky N. Reduced expression of the immediate-early protein IE0 enables efficient replication of Autographa californica multiple nucleopolyhedrovirus in poorly permissive Spodoptera littoralis cells [J]. J Virol, 2003, 77(1) : 535-545.
  • 5张琦.杆状病毒DNA甲基化及其在病毒复制过程中的作用初探[D].上海:复旦大学,2012.
  • 6Choi J, Guarino L A. Expression of the IE1 transactivator of Autographa californica nuclear polyhedrosis virus during viral infection [J]. ViroLogy, 1995, 209(1): 99-107.
  • 7Doerfler W. In pursuit of the first recognized epigenetic signal-DNA methylation[J]. Epigenetics, 2008, 3: 125-133.
  • 8Hoelzer K, Shackelton L A, Parrish C R. Presence and role of cytosine methylation in DNA viruses of animals [J]. Nucleic Acids Res, 2008, 36: 2825-2837.
  • 9Kaur P, Paliwal A, Durantel D, et al. DNA methylation of hepatitis B virus (HBV) genome associated with the development of hepatocellular carcinoma and occult HBV infection [J]. J Infect Dis, 2010, 202: 700-704.
  • 10Vivekanandan P, Daniel H D, Kannangai R, et al. Hepatitis B virus replication induces methylation of both host and viral DNA [J]. J Virol, 2010, 84(9): 4321-4329.

共引文献2

同被引文献11

  • 1Lyko F, Brown R. DNA methyhmnsferase inhibitors and the development of epigenetic cancer therapies[J]. Nat Cancer las, 2005, 97 (20) : 1498- 1506.
  • 2Eric W, Jennifer K, Jean-Pierre A, et al. Phase II trial of DNA methyltransferase 1 inhibition with the antisense oligonucleotide MG98 in patients with metastatic renal carcinoma: a national cancer institute of Canada clinical trials group investigational new drug study[J]. In- vest New Drugs, 2006, 24(2):159-167.
  • 3Gryn J, Zeigler Z R, Shadduck R K, et al. Treatment of myelodysplastic syndromes with 5-azacytidine[J]. Leuk Res, 2002, 26(10) : 893 - 897.
  • 4Momparler R L, Momparler L F, Samson J. Comparison of the antileukemic activity of 5-aza-2-deoxycytidine, 1-beta-D-ara-binofurano- sylcytosine and 5-azacyti -dine against IA210 leukemia[J], l.euk Res, 1984, 8 (6): 1043 - 1049.
  • 5Kenney S C, Mertz J E. Regulation of the latent-lyric switch in Epstein-Barr virus[J]. Semin Cancer Biol. 2014, 26(6) t 60 - 68.
  • 6Zhang X, Hou J, Lu M. Regulation of hepatitis B virus replication by epigenetic mechanisms and microRNAs[J]. Front Genet. 2013, 4:202.
  • 7Shen L, Kantarjian H, Guo Y, et al. DNA methylation predicts survival and response to therapy in patients with myelodysplastic syn dromes[J]. J Clin Oncol, 2010, 28(4) : 605 -613.
  • 8Oki Y, Aoki E, ]ssa JP, et al. Decitabine-Bedside to bench[J]. Critical Reviews in Oncology/Hematology, 2007, 61(2) : 140 - 152.
  • 9Wang X, Yin J, Zhong J, et al. DNA methyltransferase inhibitors increase baculovirus-mediated gene expression in mammalian cells when applied before infection[J]. Anal. Biochem, 2010, 396:322 -324.
  • 10O'Reilly D R, Miller L K, Luckow V A. Baculovirus expression vectors, a laboratory manual[M] New York: W. H. Freeman and Company, 1992.

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复旦学报（自然科学版）
2015年 第1期

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