慢性丙型肝炎病毒感染患者血清IL-35表达水平及其意义

IL-35 expression levers in serum and its significance in patients with chronic hepatitis C virus infection

目的：通过检测慢性丙型肝炎病毒（HCV）感染者血清中白细胞介素-35（IL-35）的表达水平,探讨IL-35在HCV持续感染致病机制中的作用。方法：用酶联免疫吸附试验（ELISA）法检测263例慢性丙型肝炎患者和117例健康体检者的血清IL-35水平,并对不同临床诊断的HCV患者间的IL-35水平进行比较分析,同时分析IL-35与ALT、AST、TBIL、ALB和HCVRNA的相关性。结果：HCV感染者血清IL-35水平明显增高,高于健康对照者,其中HCV相关的肝癌IL-35水平最高3926.1（297.2-4129.1）pg/ml,明显高于肝硬化2497.1（117.2-3491.3）pg/ml和慢性肝炎组1987.6（89.69-2987.3）pg/ml。结论：HCV感染者血清中IL-35水平明显升高,提示IL-35在HCV持续感染的免疫致病机制中起着一定作用,为临床诊断和治疗HCV感染提供了一种思路。

Objective：Through the detection of chronic hepatitis C virus（HCV）infection interleukin in serum vitamin-35（IL-35）expression levels of IL-35 inpersistent HCV infection,to explore the pathogenesis mechanism.Methods：With the method of enzyme linked immunosorbent assay（ELISA）method was used to detect the serum IL-35 level in 263 patients with chronic hepatitis C patients and 117 healthy,and the different clinical diagnosed HCV patients and the level of IL-35 were compared and analyzed,and the analysis of the IL-35 and the ALT,AST,TBIL,the correlation between ALB and HCVRNA.Results ：The serum IL-35 levels of HCV infection increased significantly,higher than that of healthy controls group,where HCV related hepatocellular carcinoma IL-35 highest level in 3926.1（297.2-4129.1）pg/ml was significantly higher than that in liver cirrhosis,2497.1（117.2-3491.3）pg/ml and chronic hepatitis B group was 1987.6（89.69-2987.3）pg/ml.Conclusion：The level of serum IL-35 is significantly increased in conclusion HCV infection,suggesting that IL-35 plays a role in the immune pathogenesis of HCV persistent infection,and provides a new idea for clinical diagnosis and treatment of the treatment of HCV infection.