肺腺癌组织中表皮生长因子表达与树突状细胞和调节性T细胞关系的研究

Investigation of the relationship between VEGF,DC and Treg cells in human lung adenocarcinoma tissues

目的探讨肺腺癌组织中表皮生长因子(VEGF)表达与树突状细胞(DC)及调节性T细胞(Treg)的关系。方法选取2000年1月至2012年12月间行手术治疗的55例肺腺癌患者术后组织标本,以及其中16例患者的癌旁正常组织。采用免疫组化方法检测癌组织和癌旁组织中VEGF、CD1a(不成熟DC表达阳性)和CD4的表达情况,分析其与患者临床特征的关系。结果 16例肺腺癌组织中VEGF的表达明显高于癌旁正常组织,差异有统计学意义(P<0.05);癌组织中CD1a DC表达[(5.59±3.71)%]明显高于癌旁正常组织[(1.39±0.61)%],差异有统计学意义(P<0.05)。癌组织中CD4T细胞阳性比例[(6.62±4.27)%]显著高于癌旁正常组织[(0.43±0.46)%],差异有统计学意义(P<0.05)。55例肺腺癌患者癌组织中,VEGF阳性45例,阳性率为81.8%。VEGF阴性和阳性患者中CD1a+DC表达差异无统计学意义(P>0.05),VEGF阴性和阳性患者中CD+4细胞表达差异无统计学意义(P>0.05)。CD1a+DC与CD4+细胞比例呈正相关(r=0.306,P=0.01)。CD1a和CD4的表达与患者性别、年龄、肿瘤大小、临床分期、淋巴结转移情况无关。结论肺腺癌组织中VEGF、不成熟DC和Treg在癌组织中的表达明显高于癌旁正常组织。不成熟DC和Treg数量与VEGF表达无关,而不成熟DC数量与Treg数量呈正相关。

Objective This study aimed to investigate the relationship between VEGF,DC and Treg in human lung adenocarcinoma. Methods From January 2000 to December 2012,55 cases of lung adenocarcinoma paraffin-embedded tissues were collected,and in these patients,16 cases of lung adenocarcinoma paraffin-embedded tissue and surrounding normal tissues were collected. Immunohistochemistry was used to detect the expression of VEGF,CD1a（ positive expression of immature DC） and CD4. The association of them were analyzed. Results In the 16 cases,positive expression rate of VEGF in the tumor tissue was higher than that in surrounding normal tissue,the difference was significant（ P〈0. 05）. The expression of CD1a＋DC in lung adenocarcinoma tissue（ 5. 59 ± 3. 71） was higher than that in surrounding normal tissue（ 1. 39 ± 0. 61）,the difference was significant（ P〉0. 05）. The percentage of CD4 positive T cell in lung adenocarcinoma tissue [（ 6. 62 ± 4. 27） %]was higher than that in surrounding normal tissue [（ 0. 43 ±0. 46% ],the difference was significant（ P〈0. 05）. In 55 cases of lung adenocarcinoma tissue,VEGF positivity was detected in 81. 8%（ 45 /55） cases. CD1 a positive cells and the percentage of CD4 positive T cell had no significant difference between VEGF positive and negative patients（ P〈0. 05）. There was a positive correlation between the expression of CD1a＋DC and percent of CD＋4Tregs in lung adenocarcinoma tissue（ r = 0. 306,P = 0. 01）. The expression of VEGF,CD1 a,CD4were not related to patient＇s gender,age,tumor size,clinical stage and lymphatic metastasis. Conclusions The expression of VEGF,immature DC,and Treg There had significant difference in lung adenocarcinoma tissue and surrounding normal tissue. Positive cells of immature DC and Treg had no significant difference in VEGF positive and negative patients.There was a positive correlation between the immature DC and Treg.