白芍总苷体外抗氧化活性研究

Antioxidant activities of total glucosides of paeony in vitro

目的研究白芍总苷体外对不同化学体系和生物体系的抗氧化活性。方法通过Fenton反应生成·OH自由基,采用DPPH·自由基,观察不同质量浓度的白芍总苷溶液对化学体系自由基的清除作用。利用邻二氮菲-Fe2+-H2O2产生的·OH自由基造成红细胞膜破裂,以及肝、脑细胞脂质的过氧化,观察不同质量浓度的白芍总苷溶液对生物体系自由基的清除作用。结果白芍总苷对·OH有一定的清除作用,其IC50为0.62 g/L。白芍总苷对DPPH·自由基有较强的清除作用,IC50为6.6 mg/L;对·OH自由基引发的红细胞膜破裂有一定的抑制作用,其IC50为30.17 mg/L,对肝、脑匀浆脂质过氧化有一定的抑制作用,其IC50分别为6.9、16.3 mg/L。结论白芍总苷具有清除自由基作用,在不同体外实验体系中抗氧化量效关系存在差异。

Objective To study antioxidant activities in vitro of total glucosides of paeony（TGP） on various chemical system and biological system. Methods Hydroxyl radical（·OH） was generated by Fenton reaction and DPPH· radicals were used. Scavenging effects of various concentrations of TGP on free radicals from different chemical antioxidant systems were evaluated. Red cell membrane rupture and the liver or brain homogenate lipid peroxidation caused by OH from phenanthridine- Fe^2＋- H2O2 system were used to evaluate scavenging effects of various concentrations of TGP on free radicals from different chemical biological systems. Results TGP had a certain effect to scavenge ·OH free radical with the IC50 value of 0.62 g/L. TGP also had stronger effect to scavenge DPPH· free radical with the IC50 value of 6.6 mg/L, and had inhibition of red cell membrane rupture with the IC50 value of 30.17 mg/L, and lipid peroxidation with the IC50 value of 6.9, 16.3 mg/L, respectively. Conclusion TGP has antioxidant abilities, and there were differences of antioxidant activity potency among differ experiment systems.