超声提取韭菜籽生物碱工艺及其活性研究

Study on ultrasonic extraction and activity of alkaloids from allium tuberosum seed

对韭菜籽生物碱的超声提取工艺进行了研究,在提取溶剂、提取溶剂浓度、提取温度、提取时间、料液比等单因素的基础上,研究了韭菜籽生物碱的超声提取法,对料液比、温度、时间、提取次数等提取因素进行优化,结果表明通过超声提取法的最佳提取工艺条件为料液比是1∶45,温度是60℃,时间为40min,功率确定为200W,在该条件下所测得的韭菜籽生物碱的含量为93μg/m L。对韭菜籽生物碱·OH的清除能力和对K3Fe(CN)6的还原性进行分别测定,结果表明韭菜籽生物碱浓度与其·OH的清除及对铁氰化钾的还原能力能力呈线性正相关。

The ultrasonic extraction and activity of alkaloids from Allium tuberosum seed were studied. The effects of different factors, such as extraction solvent, extraction solvent concentration, extraction temperature, extraction time, ratio of solid to liquid and extraction times on alkaloids yield of Allium tuberosum seed were discussed by single factor test. It made a study on the ultrasonic extraction, and the ratio of material to liquid, temperature, time and the power of extraction were screened and orthogonal test were applied. The results showed that the ultrasonic extraction had the optimum conditions were as follows： the ratio of solid to liquid was 1 ： 45, the extraction temperature was 60℃, the extraction time was 40 minutes, and the power of extraction was 200W. At this condition, we can get a yield of 93μg/mL of total alkaloids in Chinese chive seed. Ability to scavenge· OH and reduce K3Fe （CN）6 were determined. The results are as follows： It has a linear correlation between the scavenging ability to ·OH, reduction to K3Fe （CN）6 and the concentration of alkaloids.