苍柏湿毒清对解脲支原体感染小鼠IL-2的影响

Effect of Cangbaishiduqing on IL-2 level of mice infected with Ureaplasma urealyticum

目的:观察苍柏湿毒清及其拆方对解脲支原体感染BALB/c小鼠血清IL-2的影响。方法:采用苯甲酸雌二醇预处理,将解脲支原体血清4型菌液接种于BALB/c小鼠阴道,给予苍柏湿毒清低剂量、高剂量及其拆方预防性、治疗性干预,采用ELASA法检测小鼠血清IL-2水平。结果:苍柏湿毒清低剂量中药预防组提高血清IL-2水平高于其他各组(P<0.05);中药预防组、中药治疗组提高血清IL-2水平均高于模型组(P<0.05)。清热解毒组、健脾利湿组提高血清IL-2值高于正常组(P<0.05),但与模型组差异不显著(P>0.05)。结论:苍柏湿毒清在预防、治疗解脲支原体感染疾病方面均有作用;全方组优于拆方组。可能是通过清热解毒、健脾利湿药物提高血清IL-2水平进行免疫调节。

Objectives： To explore the effect of Cangbaishiduqing and that of its subdivisions on serum IL-2 level of BALB / c mice infected by Ureaplasma urealyticum（ Uu）. Method： Pretreated by estradiol benzoate,female BALB / c mice were infected by UU. Prevented and treated by low dose,high dose of Cangbaishiduqing and its subdivisions. ELISA method was used to test the level of serum IL- 2. Results： The serum IL- 2 level of the prevented low dose of Cangbaishiduqing was significantly higher than the other groups（ P〈0. 05）. The serum IL- 2level of prevented groups and treated groups were higher than the model groups（ P〈0. 05）. The serum IL- 2 level of group J（ the clearing heat and detoxifcation group） and group K（ the nourishing spleen and eliminating dampness group） were significantly higher than the normal group（ P〈0. 05）,but without significant difference from that of the model group. Conclusions： Cangbaishiduqing is effective in the prevention and treatment of infection caused by Uu,with better effect for the Cangbaishiduqing group than the subdivisions group. The medicine,through clearing heat and detoxifcation and nourishing spleen,may improve the level of serum IL- 2 to activate the immune system.