慢性乙型肝炎舌红苔黄和舌淡苔白不同舌象的尿代谢组研究

Urinary metabolic signatures of chronic hepatitis B patients with different tongue manifestations

目的:探索慢性乙型肝炎舌红苔黄和舌淡苔白不同舌象者的尿代谢差异指标,为中医舌象生物学物质基础微观辨证提供证据。方法:采用气相色谱/质谱联用(GC/MS)技术方法获取慢性乙型肝炎舌红苔黄和舌淡苔白不同舌象者的尿液样本代谢指纹谱,用无监督的学习模式进行多变量统计分析,观察不同组别的人群之间是否存在"自然"的分类结构。利用有监督的学习模式进行数据分类模型的建立和检验,寻找造成样本聚集和离散的主要差异变量。利用商业化的代谢物谱库以及标准品数据库,进行物质鉴定。结果:慢性乙型肝炎舌红苔黄和舌淡苔白者在有监督的学习模式下具有良好的分开趋势,慢乙肝不同舌象者较健康者的差异代谢物谱主要与能量代谢、氨基酸代谢、核苷酸代谢以及肠道菌群代谢相关。结论:舌象是机体变化的重要窗口,不同舌象的外在表观潜在体内的代谢差异。

Objective： Exploring the urinary metabolic profiling of chronic hepatitis b（ CHB） patients with different tongue manifestations,the red tongue with yellow tongue coating and the pale tongue with white tongue coating,to demonstrate the potential objective material basis. Methods： The urinary metabolic profiling was detected based on gas chromatography coupled to mass spectrometry（ GC / MS）,multivariate data was analyzed,the unsupervised segregation and supervised segregation analysis were checked,and the potential markers of interest were extracted and confirmed. Results： Distinct clustering in metabolic profiles were observed by supervised segregation analysis in different tongue groups,based on the variable of importance（ VIP） in the project values,significantly changed metabolites were identified. These changes were related to the disturbance in energy metabolism,amino acid metabolism,nucleotide metabolism,and gut microflora metabolism. Conclusion： This study demonstrated that tongue manifestation may have objective material basis,and closely related to the condition of the body organs function.